JCRB1033 NOZ

Cell information

Important Notice(s)

On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1033 Cell Name   NOZ
Profile   Human gallbladder carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   48
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   peritonitis carcinomatosa. Gallbladder carcinoma of adenocarcinoma tubular, moderately differentiated. AFP 420ng/ml, CEA 1.3 ng/ml. Metastasis   Yes
Tissue Metastasized   ascites Genetics   No. of chromosome = 46.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Dt=48 hrs. Plating efficiency is 14-19%. Cells are transplantable to nude mice and the morphology of the transplanted tumor was similar to the original one.
Classify   tumor Established by   Honma,S. & Nagamori,S.
Registered by   Nagamori,S. Regulation for Distribution   Require negotiation and agreement from Dr.Nagamori before shipping.
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. Year   2001
Medium   Williams'E medium with 10% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   moderately differentiated adenocarcinoma.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3079
Reference
Pubmed id:2543327 [Combination therapy of hyperthermia and other methods in liver and bile tract cancers--evaluation of these methods using cancer cell lines in vitro].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1905-12


Research results by users. Click!
Pubmed id:24655726 Oridonin induces apoptosis and cell cycle arrest of gallbladder cancer cells via the mitochondrial pathway.
Bao R,Shu Y,Wu X,Weng H,Ding Q,Cao Y,Li M,Mu J,Wu W,Ding Q,Tan Z,Liu T,Jiang L,Hu Y,Gu J,Liu Y
BMC Cancer. 2014 Mar 21;14():217
Pubmed id:25165862 Schisandrin B induces apoptosis and cell cycle arrest of gallbladder cancer cells.
Xiang SS,Wang XA,Li HF,Shu YJ,Bao RF,Zhang F,Cao Y,Ye YY,Weng H,Wu WG,Mu JS,Wu XS,Li ML,Hu YP,Jiang L,Tan ZJ,Lu W,Liu F,Liu YB
Molecules. 2014 Aug 27;19(9):13235-50
Pubmed id:24966896 miR-1 and miR-145 act as tumor suppressor microRNAs in gallbladder cancer.
Letelier P,García P,Leal P,Álvarez H,Ili C,López J,Castillo J,Brebi P,Roa JC
Int J Clin Exp Pathol. 2014;7(5):1849-67
Pubmed id:25010679 Forkhead box L1 is frequently downregulated in gallbladder cancer and inhibits cell growth through apoptosis induction by mitochondrial dysfunction.
Qin Y,Gong W,Zhang M,Wang J,Tang Z,Quan Z
PLoS One. 2014;9(7):e102084
Pubmed id:25090123 Cordycepin induces S phase arrest and apoptosis in human gallbladder cancer cells.
Wang XA,Xiang SS,Li HF,Wu XS,Li ML,Shu YJ,Zhang F,Cao Y,Ye YY,Bao RF,Weng H,Wu WG,Mu JS,Hu YP,Jiang L,Tan ZJ,Lu W,Wang P,Liu YB
Molecules. 2014 Jul 31;19(8):11350-65
Pubmed id:25277242 Expression of the RIP-1 gene and its role in growth and invasion of human gallbladder carcinoma.
Zhu G,Chen X,Wang X,Li X,Du Q,Hong H,Tang N,She F,Chen Y
Cell Physiol Biochem. 2014;34(4):1152-65
Pubmed id:24568162 Baicalin induces apoptosis of gallbladder carcinoma cells in vitro via a mitochondrial-mediated pathway and suppresses tumor growth in vivo.
Shu YJ,Bao RF,Wu XS,Weng H,Ding Q,Cao Y,Li ML,Mu JS,Wu WG,Ding QC,Liu TY,Jiang L,Hu YP,Tan ZJ,Wang P,Liu YB
Anticancer Agents Med Chem. 2014;14(8):1136-45
Pubmed id:25154789 Tumor necrosis factor-α promotes the lymphangiogenesis of gallbladder carcinoma through nuclear factor-κB-mediated upregulation of vascular endothelial growth factor-C.
Du Q,Jiang L,Wang X,Wang M,She F,Chen Y
Cancer Sci. 2014 Oct;105(10):1261-71
Pubmed id:24329682 Inhibitory effects of deleted in liver cancer 1 gene on gallbladder cancer growth through induction of cell cycle arrest and apoptosis.
Qin Y,Chu B,Gong W,Wang J,Tang Z,Shen J,Quan Z
J Gastroenterol Hepatol. 2014 May;29(5):964-72
Pubmed id:24756644 UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest.
Qin Y,Wang J,Gong W,Zhang M,Tang Z,Zhang J,Quan Z
Oncol Rep. 2014 Jun;31(6):2635-43
Pubmed id:24217644 Role of aquaporin-5 in gallbladder carcinoma.
Sekine S,Shimada Y,Nagata T,Sawada S,Yoshioka I,Matsui K,Moriyama M,Omura T,Osawa S,Shibuya K,Hashimoto I,Watanabe T,Hojo S,Hori R,Okumura T,Yoshida T,Tsukada K
Eur Surg Res. 2013;51(3-4):108-17
Pubmed id:23830367 Combination chemotherapy of nafamostat mesylate with gemcitabine for gallbladder cancer targeting nuclear factor-κB activation.
Iwase R,Haruki K,Fujiwara Y,Furukawa K,Shiba H,Uwagawa T,Misawa T,Ohashi T,Yanaga K
J Surg Res. 2013 Sep;184(1):605-12
Pubmed id:22835954 Synergic effect of photodynamic therapy using talaporfin sodium with conventional anticancer chemotherapy for the treatment of bile duct carcinoma.
Nonaka Y,Nanashima A,Nonaka T,Uehara M,Isomoto H,Abo T,Nagayasu T
J Surg Res. 2013 May;181(2):234-41
Pubmed id:22071224 Vascular endothelial growth factor-D promotes growth, lymphangiogenesis and lymphatic metastasis in gallbladder cancer.
Lin W,Jiang L,Chen Y,She F,Han S,Zhu J,Zhou L,Tang N,Wang X,Li X
Cancer Lett. 2012 Jan 28;314(2):127-36
Pubmed id:22201822 SNCG gene silencing in gallbladder cancer cells inhibits key tumorigenic activities.
Han S,She F,Wang D,Yao X,Jiang L,Chen Y
Front Biosci (Landmark Ed). 2012 Jan 1;17():1589-98
Pubmed id:22843894 Establishment and characterization of a new human gallbladder carcinoma cell line.
Sekine S,Shimada Y,Nagata T,Moriyama M,Omura T,Yoshioka I,Hori R,Matsui K,Sawada S,Okumura T,Yoshida T,Tsukada K
Anticancer Res. 2012 Aug;32(8):3211-8
Pubmed id:22320835 Adenovirus-mediated gene transfer of tissue factor pathway inhibitor-2 inhibits gallbladder carcinoma growth in vitro and in vivo.
Qin Y,Zhang S,Gong W,Li J,Jia J,Quan Z
Cancer Sci. 2012 Apr;103(4):723-30
Pubmed id:21360081 Advantages of laserphyrin compared with photofrin in photodynamic therapy for bile duct carcinoma.
Nonaka T,Nanashima A,Nonaka M,Uehara M,Isomoto H,Nonaka Y,Nagayasu T
J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):592-600
Pubmed id:20464561 Novel photodynamic therapy against biliary tract carcinoma using mono-L: -aspartyl chlorine e6: basic evaluation for its feasibility and efficacy.
Kasuya K,Shimazu M,Suzuki M,Kuroiwa Y,Usuda J,Itoi T,Tsuchida A,Aoki T
J Hepatobiliary Pancreat Sci. 2010 May;17(3):313-21
Pubmed id:20651359 Analysis of apoptotic effects induced by photodynamic therapy in a human biliary cancer cell line.
Nonaka T,Nanashima A,Nonaka M,Uehara M,Isomoto H,Asahina I,Nagayasu T
Anticancer Res. 2010 Jun;30(6):2113-8
Pubmed id:20734221 Vascular endothelial growth factor-C promotes the growth and invasion of gallbladder cancer via an autocrine mechanism.
Chen Y,Jiang L,She F,Tang N,Wang X,Li X,Han S,Zhu J
Mol Cell Biochem. 2010 Dec;345(1-2):77-89
Pubmed id:19209029 Schedule-dependent synergistic interaction between gemcitabine and oxaliplatin in human gallbladder adenocarcinoma cell lines.
Makiyama A,Qin B,Uchino K,Shibata Y,Arita S,Isobe T,Hirano G,Kusaba H,Baba E,Akashi K,Nakano S
Anticancer Drugs. 2009 Feb;20(2):123-30
Pubmed id:18204862 Upregulation of topoisomerase IIalpha expression in advanced gallbladder carcinoma: a potential chemotherapeutic target.
Washiro M,Ohtsuka M,Kimura F,Shimizu H,Yoshidome H,Sugimoto T,Seki N,Miyazaki M
J Cancer Res Clin Oncol. 2008 Jul;134(7):793-801
Pubmed id:17235714 Antitumor effect of gemcitabine on orthotopically inoculated human gallbladder cancer cells in nude mice.
Mita Y,Ajiki T,Kamigaki T,Okazaki T,Hori H,Horiuchi H,Hirata K,Fujita T,Fujimori T,Kuroda Y
Ann Surg Oncol. 2007 Apr;14(4):1374-80
Pubmed id:15855925 Oncolytic adenoviral therapy in gallbladder carcinoma.
Tekant Y,Davydova J,Ramirez PJ,Curiel DT,Vickers SM,Yamamoto M
Surgery. 2005 May;137(5):527-35
Pubmed id:15743030 A MEK inhibitor (U0126) markedly inhibits direct liver invasion of orthotopically inoculated human gallbladder cancer cells in nude mice.
Horiuchi H,Kawamata H,Furihata T,Omotehara F,Hori H,Shinagawa Y,Ohkura Y,Tachibana M,Yamazaki T,Ajiki T,Kuroda Y,Fujimori T
J Exp Clin Cancer Res. 2004 Dec;23(4):599-606
Pubmed id:12963974 A MEK inhibitor (U0126) prolongs survival in nude mice bearing human gallbladder cancer cells with K-ras mutation: analysis in a novel orthotopic inoculation model.
Horiuchi H,Kawamata H,Fujimori T,Kuroda Y
Int J Oncol. 2003 Oct;23(4):957-63
Pubmed id:2543327 [Combination therapy of hyperthermia and other methods in liver and bile tract cancers--evaluation of these methods using cancer cell lines in vitro].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1905-12
Pubmed id:3154020 [Establishment and characterization of a human gall bladder carcinoma cell line NOZ].
Homma S,Hasumura S,Nagamori S,Kameda H
Hum Cell. 1988 Mar;1(1):95-7
Pubmed id:none バイオテクノロジー素材としてのヒト癌細胞 (1)。胆嚢癌NOZ。蛋白質。核酸
永森静志
蛋白質・核酸・酵素 33, 2605-2606 (1988)
Pubmed id:none Morphological changes in human gall bladder carcinoma cell line-NOZ due to epirubicin and doxorubicin
Masaaki Kawada, Hideki Aizaki, Kenichi Fukaya, Minoru Niiya, Tomokazu Matsuura, Hajime Sujino, Satoshi Hasumura, Seishi Nagamori, Gotaro Toda
Medical Electron Microscopy 27, 175-177 (1994)
Pubmed id:none 閉塞性黄疸に対するPTCD胆汁細胞診とK-ras点突然変異の解析
味木 徹夫, 藤盛 孝博, 塚本 龍子, 渥美 亜紀子, 小野山 裕彦, 前田 盛, 斎藤 洋一
日本臨床細胞学会雑誌 34(3), 424-428 (1995)
Pubmed id:none 微小管を標的とした抗癌剤のヒト胆嚢癌細胞に対する効果に関する基礎的研究
柴本 由香, 高木 一郎
胆道 14(1), 42-50 (2000)

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LOT Information

Cell No.   JCRB1033 Cell Name   NOZ
LOT No.   08052009 Lot Specification   distribution
Medium   William's E medium with10% heat inactivated fetal bovine serum(Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%(GIBCO) trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.5x10^6
Viability at cell freezing (%)   99.1 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,12
D7S820:10,11
D16S539:9,11
VWA:19
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1033 Cell Name   
LOT No.   09162008 Lot Specification   distribution
Medium   William's medium E with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30 hrs Cell Number in Vial (cells/1ml)   4.8 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - William's medium E CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1033 Cell Name   NOZ
LOT No.   02122003 Lot Specification   distribution
Medium   William's E medium with 10% fetal calf serum(Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA. Medium change twice a week and subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,12
D7S820:10,11
D16S539:9,11
VWA:19
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
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