JCRB1039 SKN-3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1039 Cell Name   SKN-3
Profile   Human oral squamous carcinoma established from xenotransplantation in mice. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   oral
Case history   oral squamous carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells were estabished through xenotransplanted tumor in nude mice
Classify   tumor Established by   Yanagihara,K.
Registered by   Yanagihara,K. Regulation for Distribution   
Comment    Year   2003
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   1.7 x 10^6 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   oral cavity
Virus Summary   tested Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested, GAPDH = positive control]
Tissue Type   squamous carcinoma
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LOT Information

Cell No.   JCRB1039 Cell Name   
LOT No.   09012008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   4 - 7 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability (%)   97 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Additional information   
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