JCRB1045 OVMANA

Cell information

Important Notice(s)

JCRB1045 OVMANA: weak to freeze-thawing.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1045 Cell Name   OVMANA
Profile   Human cell line derived from ovarian tumour. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   51
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian carcinoma, stage IV, CDDP-IP(3) treatment done previously. Metastasis   No
Tissue Metastasized    Genetics   ER(-), PR(-) / tumour markers, CA125, SLX, TPA(o).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells are transplantable to mouse hypodermis but not to abdominal.
Classify   tumour Established by   Gorai,I. et.al.
Registered by   Gorai,I. Regulation for Distribution   
Comment   Td=26.1 hours Year   2002
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage   5 x 10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   clear cell adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3111
Reference
Pubmed id:11121861Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells.
Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S
Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.
Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H
Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties.
Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H
Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice.
Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M
Clin Exp Metastasis. 1995 Mar;13(2):89-96

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LOT Information

Cell No.   JCRB1045 Cell Name   OVMANA
LOT No.   09042012 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   Slow (approx. 55-60 hrs.) Cell Number in Vial (cells/1ml)   8.5 x 10^5
Viability at cell freezing (%)   75.5 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:8,9
D7S820:10,11
D16S539:10
VWA:15,16
TH01:7,9
AM:X
TPOX:8
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1045 Cell Name   OVMANA
LOT No.   04082003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.1 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   83.8 Antibiotics Used   free
Passage Number   P51 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   Cells were unique. Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1045 Cell Name   
LOT No.   02042008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). The vial label (OMAMA) is wrong. Correct cell line name is OVMANA). Temperature   37 C
Cell Density at Seeding   8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA for 5 - 10 min at 37 C
Doubling Time   50 - 60 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P56 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1045 Cell Name   OVMANA
LOT No.   05022017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat.# 172012) Temperature   37 C
Cell Density at Seeding   1.3 - 6.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   85.0 Antibiotics Used   free
Passage Number   P58 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO (<--important), 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   85.0
Additional information   
Cell No.   JCRB1045 Cell Name   OVMANA
LOT No.   10022018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.9 - 4.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   approx. 51 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80.0
Additional information   
Cell No.   JCRB1045 Cell Name   OVMANA
LOT No.   08102021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   6.3 - 8.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 61 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   99.2
Additional information   
Cell No.   JCRB1045 Cell Name   OVMANA
LOT No.   10262021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   7.7 - 8.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   44 - 171 hrs. Cell Number in Vial (cells/1ml)   8.3 x 10^5
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88.4
Additional information   
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