JCRB1048 OVTOKO

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1048 Cell Name   OVTOKO
Profile   Human cell line derived from ovarian tumour. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   78
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian carcinoma, stage IIIb, CAP(5) treatment done previously. Metastasis   Yes
Tissue Metastasized   spleen metastasis Genetics   ER(-), PR(-) / tumour markers not identified.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells are transplantable to both mouse hypodermis and abdominal.
Classify   tumour Established by   Gorai,I. et.al.
Registered by   Gorai,I. Regulation for Distribution   
Comment   Td=26.1 hours Year   2002
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage   5 x 10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   spleen metastatic focus
Tissue Type   clear cell adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3117
Reference
Pubmed id:11121861Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells.
Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S
Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.
Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H
Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties.
Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H
Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice.
Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M
Clin Exp Metastasis. 1995 Mar;13(2):89-96

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LOT Information

Cell No.   JCRB1048 Cell Name   
LOT No.   06082011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1.1 - 7.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin.
Doubling Time   approx. 1 days Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   92.1 Antibiotics Used   free
Passage Number   P61 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1048 Cell Name   
LOT No.   09122007 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum(lot:GIBCO484375) Temperature   37C
Cell Density at Seeding   5.0 x 10^4cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   30hr Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P62 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1048 Cell Name   OVTOKO
LOT No.   03122003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.8 x 10^4 cells/sq.cm. Methods for Passages   Harvest cells after treatment with 0.05% trypsin for 5-10min. Medium change twice a week. Subculture every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P58 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1048 Cell Name   OVTOKO
LOT No.   10092014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 2.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P61 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:8,12
D7S820:9,10
D16S539:11,12
VWA:14,17
TH01:6,9.3
AM:X
TPOX:8,12
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
Cell No.   JCRB1048 Cell Name   OVTOKO
LOT No.   04132017 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   3.9 - 7.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P62 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   98.4 Additional information   
Cell No.   JCRB1048 Cell Name   OVTOKO
LOT No.   06072021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.5 - 1.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 5-10 min.
Doubling Time   24 - 27 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   P62 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   99.3 Additional information   
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