JCRB1075.0 IM95

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1075.0 Cell Name   IM95
Profile   Human gastric cancer cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   63
Identity   available Tissue for Primary Cancer   stomach
Case history   moderately differentiated adenocarcinoma of stomach Metastasis   
Tissue Metastasized    Genetics   Modal chromosome number = 46.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Td=25 hrs. CEA related antigen observed. HGF production and tumorigenesity is maintained in the cell line.
Classify   tumor Established by   Iwai,Y. & Matsuda,M.
Registered by   Iwai,M. Regulation for Distribution   
Comment    Year   2003
Medium   Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal bovine serum and 10mg/L insulin. Methods for Passages   Cells were harvested after the treatment with 0.3% trypsin and 0.03% EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   stomach
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   stomach cancer
Link
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240155
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LOT Information

Cell No.   JCRB1075.0 Cell Name   
LOT No.   10132009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 4.5 g/L glucose (high glucose) 10 ug/ml insulin and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.3 - 4.6 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 46 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability (%)   94.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,11
D13S317:10,12
D7S820:9.3,10.3
D16S539:9,10
VWA:17,18,19
TH01:6,10
AM:X
TPOX:8,10
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB1075.0 Cell Name   
LOT No.   11162005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose; 4.5 g/L) with 10 ug/ml insulin and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   4 - 8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.2 days Cell Number in Vial (cells/1ml)   10.0 x 10^6
Viability (%)   96 Antibiotics Used   free
Passage Number   Unknown (P7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB1075.0 Cell Name   IM95
LOT No.   09302016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) and 10 mg/L insulin. Temperature   37 C
Cell Density at Seeding   0.7 - 1.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability (%)   76 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Additional information   
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