JCRB1075.1 IM95m

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1075.1 Cell Name   IM95m
Profile   Human gastric cancer cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   63
Identity   available Tissue for Primary Cancer   stomach
Case history   moderately differentiated adenocarcinoma of stomach Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Clone from the IM95. A clone from the IM95, produces higher level of HGF & VEGF.
Classify   tumor Established by   Iwai,M. & Matsuda,M.
Registered by   Iwai,M. Regulation for Distribution   
Comment    Year   2003
Medium   Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal bovine serum and 10mg/L insulin. Methods for Passages   Cells were harvested after the treatment with trypsin-EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   stomach
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   stomach cancer
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2962
Reference
Pubmed id:14609362 Cloning of a cancer cell-producing hepatocyte growth factor, vascular endothelial growth factor, and interleukin-8 from gastric cancer cells.
Iwai M,Matsuda M,Iwai Y
In Vitro Cell Dev Biol Anim. 2003 Jul-Aug;39(7):288-90

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LOT Information

Cell No.   JCRB1075.1 Cell Name   IM95m
LOT No.   08092011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose, 4.5 g/L) with 10 ug/mL insulin and 10% fetal bovine serum (FBS: GIBCO Cat.# 10099) Temperature   37 C
Cell Density at Seeding   5.2 -5.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passaged twice a week.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   6.6 x 10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,11
D13S317:10,12
D7S820:9.3
D16S539:9,10
VWA:17,18
TH01:6,10
AM:X
TPOX:8,10
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1075.1 Cell Name   
LOT No.   02062006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10 ug/ml insulin and 10% fetal bovine serum (FBS Lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   2-3 x 10^5 cells/ml Methods for Passages   Harvested with 0.25% trypsin and 0.02% EDTA (could not completely dissociate into single cell suspension). Medium renewal every 2-3 days.
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   Unknown (P6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
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