JCRB1083 KYSE180

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1083 Cell Name   KYSE180
Profile   Human well differentiated squamous cell carcinoma from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   53
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophagus cancer, T4N1Mo Stage 3, squamous cell carcinoma (SCC) Metastasis   No
Tissue Metastasized    Genetics   cyclin D1 amplification, p53 mutation
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Xenotransplantation positive.
Classify   tumor Established by   Shimada,Y.
Registered by   Shimada,Y. Regulation for Distribution   Cite reference {5368}. Company users require MTA from the originator. See additional information.
Comment   Distribution restricted to Japan(domestic use only). Year   2003
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Virus Summary   tested Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested, GAPDH = positive control]
Tissue Type   Well differenciated squamous cell carcinoma.
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1349
Reference
Pubmed id:11092977 Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon.
Pimkhaokham A,Shimada Y,Fukuda Y,Kurihara N,Imoto I,Yang ZQ,Imamura M,Nakamura Y,Amagasa T,Inazawa J
Jpn J Cancer Res. 2000 Nov;91(11):1126-33
Pubmed id:10446988 Lymph node metastasis is associated with allelic loss on chromosome 13q12-13 in esophageal squamous cell carcinoma.
Harada H,Tanaka H,Shimada Y,Shinoda M,Imamura M,Ishizaki K
Cancer Res. 1999 Aug 1;59(15):3724-9
Pubmed id:9699676 Methylation of the 5' CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas.
Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K
Cancer Res. 1998 Aug 1;58(15):3429-34
Pubmed id:9033652 Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860 Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:9816044 p53 mutation, murine double minute 2 amplification, and human papillomavirus infection are frequently involved but not associated with each other in esophageal squamous cell carcinoma.
Shibagaki I,Tanaka H,Shimada Y,Wagata T,Ikenaga M,Imamura M,Ishizaki K
Clin Cancer Res. 1995 Jul;1(7):769-73
Pubmed id:7913084 Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:8518899 Prognostic significance of cell culture in carcinoma of the oesophagus.
Shimada Y,Imamura M
Br J Surg. 1993 May;80(5):605-7
Pubmed id:1728357 Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

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LOT Information

Cell No.   JCRB1083 Cell Name   KYSE180
LOT No.   07252008 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(GIBCO)(1/1) with 2% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.9x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free (this lot was pretreated with plasmocin)
Passage Number   p715 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,11
D13S317:9,12
D7S820:9,12
D16S539:9,10
VWA:16,17
TH01:7
AM:X
TPOX:11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1083 Cell Name   KYSE180
LOT No.   08012008 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(GIBCO)(1/1) with 2% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.9x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.0x10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free (this lot was pretreated with BM cyclin 1and
Passage Number   p716 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,11
D13S317:9,12
D7S820:9,12
D16S539:9,10
VWA:16,17
TH01:7
AM:X
TPOX:11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1083 Cell Name   KYSE180
LOT No.   10222004 Lot Specification   distribution
Medium   1 to 1 mix of Ham's F12 and RPMI1640 medium containing 2% fetal calf serum. Temperature   37 C
Cell Density at Seeding   4.0 x 10^4 cells / sq. cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin about 5 min. Subculture once a week and medium change every 2-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P715 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO at final concentration. CO2 Conc.   5%
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
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