JCRB1087 KYSE270

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1087 Cell Name   KYSE270
Profile   Human squamous cell carcinoma from esophagus cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   79
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophagus cancer Metastasis   No
Tissue Metastasized    Genetics   Tumor cell line with P53 mutation. Species verified by HLA analysis.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   T2N0M0 Stage 2a. No virus infection observed.
Classify   tumor Established by   Shimada,Y.
Registered by   Shimada,Y. Regulation for Distribution   Cite reference {5368}. Company users require MTA from the originator. See additional information.
Comment   Distribution restricted to Japan(domestic use only). Year   2004
Medium   1 to 1 mixture of Ham's F12 medium and RPMI1640 medium containing 2% fetal calf serum. Methods for Passages   
Cell Number on Passage   Split ratio = 1/5. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   Well differenciated SCC
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1350
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907319
Reference
Pubmed id:11092977 Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon.
Pimkhaokham A,Shimada Y,Fukuda Y,Kurihara N,Imoto I,Yang ZQ,Imamura M,Nakamura Y,Amagasa T,Inazawa J
Jpn J Cancer Res. 2000 Nov;91(11):1126-33
Pubmed id:10446988 Lymph node metastasis is associated with allelic loss on chromosome 13q12-13 in esophageal squamous cell carcinoma.
Harada H,Tanaka H,Shimada Y,Shinoda M,Imamura M,Ishizaki K
Cancer Res. 1999 Aug 1;59(15):3724-9
Pubmed id:9699676 Methylation of the 5' CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas.
Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K
Cancer Res. 1998 Aug 1;58(15):3429-34
Pubmed id:9033652 Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860 Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:9816044 p53 mutation, murine double minute 2 amplification, and human papillomavirus infection are frequently involved but not associated with each other in esophageal squamous cell carcinoma.
Shibagaki I,Tanaka H,Shimada Y,Wagata T,Ikenaga M,Imamura M,Ishizaki K
Clin Cancer Res. 1995 Jul;1(7):769-73
Pubmed id:7913084 Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:8518899 Prognostic significance of cell culture in carcinoma of the oesophagus.
Shimada Y,Imamura M
Br J Surg. 1993 May;80(5):605-7
Pubmed id:1728357 Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

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LOT Information

Cell No.   JCRB1087 Cell Name   
LOT No.   06152005 Lot Specification   distribution
Medium   1:1 mixture of RPMI1640 medium and Ham's F12 medium with 2% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 - 9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 17 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P423 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:12
D7S820:11
D16S539:14
VWA:16,17
TH01:9
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1087 Cell Name   KYSE270
LOT No.   06072005 Lot Specification   distribution
Medium   RPMI1640 and Ham's F12 medium(1/1) with 2% heat inactivated fetal calf serum Temperature   37 C
Cell Density at Seeding   5.5X10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8x10^6
Viability at cell freezing (%)   92.9 Antibiotics Used   Free
Passage Number   P422 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, LDH and G6PD(typeB) examined. Human
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:12
D7S820:11
D16S539:14
VWA:16,17
TH01:9
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with final 10% fetal calf serum and 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1087 Cell Name   
LOT No.   11022004 Lot Specification   seed/1st distribution
Medium   1:1 mixture of RPMI1640 and Ham's F12 medium with 2% fetal bovine serum (FBS lot used; INTERGEN RB52305) Temperature   37 C
Cell Density at Seeding   4-5 x 104 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin. Subculture once a week and medium renewal every 2-3 days.
Doubling Time    Cell Number in Vial (cells/1ml)   5.4 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free. (This lot was pretreated with BM cyclin 1)
Passage Number   P420 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium containing 10% FBS and 5% DMSO. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
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