Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1135 Cell Name   KUM4
Profile   Osteoblastic cell line of mouse C3H. Other Name   
Animal   mouse Strain   C3H
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   bone marrow
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa,A.
Registered by   Umezawa,A. Regulation for Distribution   
Comment    Year   2005
Medium   Poweredby10 Methods for Passages   0.25% Trypsin - 0.53 mM EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   Bone Marrow
Tissue Type   osteoblasts

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5150
Pubmed id:15996824Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts.
Matsumoto S,Shibuya I,Kusakari S,Segawa K,Uyama T,Shimada A,Umezawa A
Biochim Biophys Acta. 2005 Aug 30;1725(1):57-63
Pubmed id:1373147Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.
Umezawa A,Maruyama T,Segawa K,Shadduck RK,Waheed A,Hata J
J Cell Physiol. 1992 Apr;151(1):197-205


LOT Information

Cell No.   JCRB1135 Cell Name   KUM4
LOT No.   11212005 Lot Specification   distribution
Medium   POWEREDBY10 Temperature   37 C
Cell Density at Seeding   2.2x10^3 cells/sq.cm Methods for Passages   Cells were harvestedtreatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   91.7 Antibiotics Used   Free
Passage Number   P2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP and LDH. Mouse
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information