Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1207 Cell Name   SV
Profile   SV40 immortalized beta-galactosidase gene deficient mouse fibroblast cell line. Human disease animal model mouse (GM1 gangliosidosis) derived cell line. Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musclus
Sex    Age   4 days-old
Identity    Tissue for Primary Cancer   skin
Case history   beta-galactosidase gene deficient cell line immortalized by SV40 introduction. Metastasis   
Tissue Metastasized    Genetics   beta-gal gene deficient
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Namba,E. and Matsuda,J.
Registered by   Higaki,E. Regulation for Distribution   
Comment    Year   2005
Medium   Dulbecco's modified Eagle's medium containing 10% fetal calf serum. Methods for Passages   Cells are harvested with standard trypsin-EDTA method. Cell storage uses DMEM(10%FCS) containing 10% DMSO.
Cell Number on Passage   Split ratio = 1/3 - 1/4. Race   
CO2 Conc.   5 % Tissue Sampling   skin (beta-gal deficient mouse)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E938
Pubmed id:14676316Chemical chaperone therapy for brain pathology in G(M1)-gangliosidosis.
Matsuda J,Suzuki O,Oshima A,Yamamoto Y,Noguchi A,Takimoto K,Itoh M,Matsuzaki Y,Yasuda Y,Ogawa S,Sakata Y,Nanba E,Higaki K,Ogawa Y,Tominaga L,Ohno K,Iwasaki H,Watanabe H,Brady RO,Suzuki Y
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15912-7
Pubmed id:11504597Galactonojirimycin derivatives restore mutant human beta-galactosidase activities expressed in fibroblasts from enzyme-deficient knockout mouse.
Tominaga L,Ogawa Y,Taniguchi M,Ohno K,Matsuda J,Oshima A,Suzuki Y,Nanba E
Brain Dev. 2001 Aug;23(5):284-7
Pubmed id:9337086Beta-galactosidase-deficient mouse as an animal model for GM1-gangliosidosis.
Matsuda J,Suzuki O,Oshima A,Ogura A,Noguchi Y,Yamamoto Y,Asano T,Takimoto K,Sukegawa K,Suzuki Y,Naiki M
Glycoconj J. 1997 Sep;14(6):729-36


LOT Information

Cell No.   JCRB1207 Cell Name   SV
LOT No.   09042007 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(Intergen Lot.RB52305) Temperature   37 C
Cell Density at Seeding   5.2x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   p13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 10% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information