JCRB1216 MT-4

Cell information

Cell type:virus production cells (View Pricing Information)

JCRB No.   JCRB1216 Cell Name   MT-4
Profile   Human leukocyte cell line established by co-cultivation with human ATL cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   Co-cultivation of ATL cells with human cord leukocytes. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoid Character   Lower HTLV-1 positive cell ratio than MT-2 and MT-3.
Classify   transformed Established by   Miyoshi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   Split ratio = 1/2 - 1/3. Race   Japanese
CO2 Conc.   5% Tissue Sampling   
Tissue Type   lymphocytes

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 +
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 + HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2632

LOT Information

Cell No.   JCRB1216 Cell Name   MT-4
LOT No.   10152007 Lot Specification   distribution
Medium   RPMI1640( GIBCO) with 10% heat inactivated fetal bovine serum (Intergen RB52305) Temperature   37 C
Cell Density at Seeding   5.7x10^5 cells/ml Methods for Passages   suspension culture
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information