JCRB1228 NCC-StC-K140

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1228 Cell Name   NCC-StC-K140
Profile   Human stomach carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   36
Identity   available Tissue for Primary Cancer   stomach
Case history   Primary stomac carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like in clusters Character   
Classify   tumor Established by   Inomata, M.
Registered by   Inomata, M. Regulation for Distribution   
Comment    Year   2006
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Simple dilution. split ratio=1/9. Subculture every 1 week.
Cell Number on Passage   1 x 10^6 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   Stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3055
Reference
Pubmed id:7499444Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid.
Inomata M,Saijo N,Kawashima K,Kaneko A,Fujiwara Y,Kunikane H,Tanaka Y
J Cancer Res Clin Oncol. 1995;121(12):729-38

Images



LOT Information

Cell No.   JCRB1228 Cell Name   NCC-StC-K140
LOT No.   03032008 Lot Specification   distribution
Medium   RPMI1640 with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2.3x10^5 cells/ml Methods for Passages   Simple dilution to 1:9 split per week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   NT
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9
D13S317:12
D7S820:11,12
D16S539:12
VWA:14
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:10
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1228 Cell Name   NCC-StC-K140
LOT No.   03222018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.0 - 1.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). The growth at the intiation of culture is slow.
Doubling Time   approx. 44 hrs. (at log phase) Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96
Additional information   
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