JCRB1317 KCL-22

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1317 Cell Name   KCL-22
Profile   Human myeloid cell line from chronic myelocytic leukemia. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   28
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   Ph1-positive chronic myeloblastic leukemia (CML). Metastasis   
Tissue Metastasized    Genetics   Ph1-positive.
Life Span   infinite Crisis PDL   
Morphology   myeloid Character   
Classify   tumor Established by   Kubonishi, I.
Registered by   Miyoshi, I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   1/2 - 1/3 Race   Japanese
CO2 Conc.   5% Tissue Sampling   pleural effusion
Tissue Type   leukemic cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2091
Reference
Pubmed id:6576961 Two new human myeloid cell lines derived from acute promyelocytic leukemia and chronic myelocytic leukemia.
Kubonishi I,Machida K,Sonobe H,Ohtsuki Y,Akagi T,Miyoshi I
Gan. 1983 Jun;74(3):319-22
Pubmed id:6321612 Establishment of a Ph1 chromosome-positive cell line from chronic myelogenous leukemia in blast crisis.
Kubonishi I,Miyoshi I
Int J Cell Cloning. 1983 Jun;1(2):105-17

Images



LOT Information

Cell No.   JCRB1317 Cell Name   KCL-22
LOT No.   12092010 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 - 3 x 10^5 cells/ml Methods for Passages   Simple dilution
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^5
Viability at cell freezing (%)   75.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   Yes
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1317 Cell Name   
LOT No.   08162008 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% heat-inactivated fetal bovine serum (Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   Simple dilution, suspension culture. Split ratio 1/2 - 1/3.
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   92.4 Antibiotics Used   fre
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Cell Banker BLC-1 (Nihon Zenyaku Ind.) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1317 Cell Name   KCL-22
LOT No.   08192008 Lot Specification   distribution
Medium   RPMI1640 medium with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   7.6x10^5 cells/ml Methods for Passages   split 1/2-1/3
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,12
D7S820:11,12
D16S539:12
VWA:14
TH01:7,9
AM:X
TPOX:8
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1317 Cell Name   KCL-22
LOT No.   09172013 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.44-7.9x10^5 cells/ml Methods for Passages   Dilution.Split 1/2-1/3
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^7
Viability at cell freezing (%)   79.3 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,12
D7S820:11,12
D16S539:12
VWA:14
TH01:7,9
AM:X
TPOX:8
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
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