JCRB1329 Squeaky

Cell information

Cell type:human iPS cells (View Pricing Information)

JCRB No.   JCRB1329 Cell Name   Squeaky
Profile   Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   14 week fetus
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc).
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   Gene Expression (OCT4, SOX2, NANOG, TERT): Positive, ALP: Positive, Immunostaining (OCT4, SOX2, NANOG, SSEA-4, Tra 1-60): Positive, Teratoma: Negative.
Classify   transformed Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2008
Medium   human ES medium (iPSellon (cardio inc.) ) Methods for Passages   Subculture once a week by mechanical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.)
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8426
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LOT Information

Cell No.   JCRB1329 Cell Name   Squeaky
LOT No.   09041013 Lot Specification   seed/distribution
Medium   iPSellon+10ng/mL FGF-2 Temperature   37 C
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number   P5+10-P5+13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   46 Chromosome Information   46,XY,der(22)t(17;22)(q21.3;p11.2)
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1329 Cell Name   Squeaky
LOT No.   03302009 Lot Specification   distribution
Medium   iPSellon Temperature   
Cell Density at Seeding    Methods for Passages   Subculture once a week by mechanical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.)
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number   P5+12 PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1329 Cell Name   Squeaky
LOT No.   04072009 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number   P5+13 PDL   NT
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing   37 C CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
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