JCRB1341 iPS-TIG120-3f7

Cell information

Cell type:human iPS cells (View Pricing Information)

JCRB No.   JCRB1341 Cell Name   iPS-TIG120-3f7
Profile   Human iPS cell line which was generated from adult human skin fibroblast cell line (JCRB0542: TIG-120). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   6
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Transgene: Human OCT3/4, SOX2, and KLF4 (in pMXs retroviral vectors)
Life Span   infinite Crisis PDL   
Morphology   human ES-like Character   
Classify   transformed Established by   Yamanaka, S.
Registered by   Yamanaka, S. Regulation for Distribution   1) Academic research purpose only. 2) Conclude a MTA with Kyoto Univ..
Comment    Year   2009
Medium   Primate ES cells medium (ReproCELL) supplemented 4 ng/mL bFGF. Methods for Passages   Cells are treated with the solution consiseted by 0.25% Trypsin, 0.1 mg/mL collagenase IV, 1mM CaCl2, and 20% KSR.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_C712
Reference
Pubmed id:22119741Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.
International Stem Cell Initiative.,Amps K,Andrews PW,Anyfantis G,Armstrong L,Avery S,Baharvand H,Baker J,Baker D,Munoz MB,Beil S,Benvenisty N,Ben-Yosef D,Biancotti JC,Bosman A,Brena RM,Brison D,Caisander G,Camarasa MV,Chen J,Chiao E,Choi YM,Choo AB,Collins D,Colman A,Crook JM,Daley GQ,Dalton A,De Sousa PA,Denning C,Downie J,Dvorak P,Montgomery KD,Feki A,Ford A,Fox V,Fraga AM,Frumkin T,Ge L,Gokhale PJ,Golan-Lev T,Gourabi H,Gropp M,Lu G,Hampl A,Harron K,Healy L,Herath W,Holm F,Hovatta O,Hyllner J,Inamdar MS,Irwanto AK,Ishii T,Jaconi M,Jin Y,Kimber S,Kiselev S,Knowles BB,Kopper O,Kukharenko V,Kuliev A,Lagarkova MA,Laird PW,Lako M,Laslett AL,Lavon N,Lee DR,Lee JE,Li C,Lim LS,Ludwig TE,Ma Y,Maltby E,Mateizel I,Mayshar Y,Mileikovsky M,Minger SL,Miyazaki T,Moon SY,Moore H,Mummery C,Nagy A,Nakatsuji N,Narwani K,Oh SK,Oh SK,Olson C,Otonkoski T,Pan F,Park IH,Pells S,Pera MF,Pereira LV,Qi O,Raj GS,Reubinoff B,Robins A,Robson P,Rossant J,Salekdeh GH,Schulz TC,Sermon K,Sheik Mohamed J,Shen H,Sherrer E,Sidhu K,Sivarajah S,Skottman H,Spits C,Stacey GN,Strehl R,Strelchenko N,Suemori H,Sun B,Suuronen R,Takahashi K,Tuuri T,Venu P,Verlinsky Y,Ward-van Oostwaard D,Weisenberger DJ,Wu Y,Yamanaka S,Young L,Zhou Q
Nat Biotechnol. 2011 Nov 27;29(12):1132-44
Pubmed id:18059259Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.
Nakagawa M,Koyanagi M,Tanabe K,Takahashi K,Ichisaka T,Aoi T,Okita K,Mochiduki Y,Takizawa N,Yamanaka S
Nat Biotechnol. 2008 Jan;26(1):101-6
Pubmed id:18035408Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Takahashi K,Tanabe K,Ohnuki M,Narita M,Ichisaka T,Tomoda K,Yamanaka S
Cell. 2007 Nov 30;131(5):861-72

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LOT Information

Cell No.   JCRB1341 Cell Name   iPS-TIG120-3f7
LOT No.   09102009 Lot Specification   distribution
Medium   culture medium for human ES cells (attachmentA-1)+FGF-2 4ng/ml Temperature   37 C
Cell Density at Seeding   1/2-1/5 split Methods for Passages   0.1% Dispase(Dispase II neutral protease,grade II,Roche)
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   p7+16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   46 Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   culture medium containing 10% DMSO CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1341 Cell Name   iPS-TIG120-3f7
LOT No.   09152009 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode   46 Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,11
D13S317:10
D7S820:10,12
D16S539:12
VWA:14,20
TH01:9,9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
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