JCRB1356 A31-1-1

Cell information

Important Notice(s)

Balb/c 3T3 A31-1に派生する細胞株の由来の問題について

The problem of origin of Balb/c 3T3 A31-1 derived cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1356 Cell Name   A31-1-1
Profile   fibroblast, subclone of Balb/3T3-A31-1, (Strain problem was found. Refer Important Notice at cell detail page.) Other Name   A31-1-1
Animal   mouse Strain   Swiss
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history   normal Metastasis   
Tissue Metastasized    Genetics   Origin of this cell line, Balb/c 3T3 A31-1, was found not to be derived from BALB 3T3 A31.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast, This cell line exhibits contact inhibition.
Classify   normal Established by   Kakunaga,T.
Registered by   Ota,T.&Tatsuka,M. Regulation for Distribution   
Comment   The FCS requires careful selection to keep quality. Year   2008
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin & 0.02% EDTA. Practically, 1/100 dilution once a week. Avoid to be confluent.
Cell Number on Passage   3 x 10^5 cells/60 mm dish Race   
CO2 Conc.   5% Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J646
Pubmed id:7394516Cell variants showing differential susceptibility to ultraviolet light--induced transformation.
Kakunaga T,Crow JD
Science. 1980 Jul 25;209(4455):505-7
Pubmed id:4311790Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
Jainchill JL,Aaronson SA,Todaro GJ
J Virol. 1969 Nov;4(5):549-53
Pubmed id:4301006Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
Aaronson SA,Todaro GJ
J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:13985244Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
J Cell Biol. 1963 May;17():299-313


LOT Information

Cell No.   JCRB1356 Cell Name   A31-1-1
LOT No.   12012009 Lot Specification   distribution
Medium   Eagle's minimum essential medium(Nissui) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.21-1.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.Practically,1/100 dilution once a week. Avoid to be cofluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.74x10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information