JCRB1423 PL521

Cell information

Important Notice(s)

POWEREDBY10 medium

不死化していないヒト体性幹細胞の分譲について(倫理的な取り扱い)

On the distribution of human non-immortalized somatic stem cells to oversea users (ethical ragulation).

Cell type:primary mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1423 Cell Name   PL521
Profile   Human placenta-derived mesenchymal stem cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   primary Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2008
Medium   Poweredby 10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-quarter of harvested cells.
Cell Number on Passage   2 x 10^5/10 cm dish Race   unknown
CO2 Conc.   5% Tissue Sampling   Placenta
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8530
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LOT Information

Cell No.   JCRB1423 Cell Name   PL521
LOT No.   08232011 Lot Specification   distribution
Medium   POWEREDBY10(within serum) with 10% fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   4.53-9.28x10^3cells/sq.cm Methods for Passages   When the cultures reached subconfluence,the cells were harvested after treatment with 0.25% trypsin and 1mM EDTA and replaced with one-quarter of harvested cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.00x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   NT
Passage Number   p3 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11,12
D7S820:10,13
D16S539:9
VWA:14
TH01:6
AM:X,Y
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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