JCRB1436 KYSE510

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1436 Cell Name   KYSE510
Profile   Human well differentiated squamous cell carcinoma cell line established from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   67-year-old
Identity   available Tissue for Primary Cancer   esophagus
Case history   well differentiated squamous cell carcinoma, stage 3 Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   T4M1N0 stage 3
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2011
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   esophagus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1354
Images



LOT Information

Cell No.   JCRB1436 Cell Name   KYSE510
LOT No.   11072011 Lot Specification   distribution
Medium   RPMI1640 and Ham's F12 medium(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/5
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.70x10^7
Viability at cell freezing (%)   92.2 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:11,12
D16S539:9
VWA:14
TH01:9
AM:X
TPOX:8
CSF1PO:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
CLOSE