JCRB1489 POLZ D2781N

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1489 Cell Name   POLZ D2781N
Profile   Human cell line derived from B cell leukemia. (MSH+, POLZ low activity, TK+/-) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   19-year-old
Identity   available Tissue for Primary Cancer   
Case history   acute lymphoblastic leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   MSH+, POLZ D2781N/D2781N, TK+/-
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Nohmi, T.
Registered by   Nohmi, T. Regulation for Distribution   Required depositor's approval.
Comment    Year   2012
Medium   RPMI 1640 + 10% heat-inactivate FBS Methods for Passages   dilution
Cell Number on Passage   twice/week, split ratio = 1:10 Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood
Virus Summary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control] Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JH09
Reference
Pubmed id:27338670 Sensitivity of human cells expressing low-fidelity or weak-catalytic-activity variants of DNA polymerase ζ to genotoxic stresses.
Suzuki T,Grúz P,Honma M,Adachi N,Nohmi T
DNA Repair (Amst). 2016 Sep;45():34-43

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LOT Information

Cell No.   JCRB1489 Cell Name   POLZ D2781N
LOT No.   09142012 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.9-9.0x10^5 cells/ml Methods for Passages   Dilution twice a week (split ratio=1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.67x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:9,13
D7S820:8,12
D16S539:9,11
VWA:15,16
TH01:8,9
AM:X,Y
TPOX:8,10
CSF1PO:14
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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