JCRB1502 MDA-MB-361-Luc#1

Cell information

Important Notice(s)

Leibovitz's L-15 medium

発光細胞 (ルシフェラーゼ発現細胞)について / Ordering Luc cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1502 Cell Name   MDA-MB-361-Luc#1
Profile   Luciferase stably expressing cell line (MDA-MB-361; Human breast carcinoma cell line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   40-year-old
Identity   available Tissue for Primary Cancer   breast
Case history    Metastasis   Yes
Tissue Metastasized   brain Genetics   wnt7h +, pMSCV-luc transfected. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   Leibovitz's L-15 Medium with 20% fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio = 1/2 - 1/6 Race   Caucasian
CO2 Conc.    Tissue Sampling   This line was isolated from a brain
Virus Summary    Virus Tested   
Tissue Type   mammary gland; breast
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LOT Information

Cell No.   JCRB1502 Cell Name   MDA-MB-361-Luc#1
LOT No.   12122012 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:11
D7S820:9,12
D16S539:11,12
VWA:17
TH01:9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1502 Cell Name   MDA-MB-361-Luc#1
LOT No.   01282013 Lot Specification   distribution2
Medium   Leivobitz's L15(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   7.2-9.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA(Split ratio=1/2-1/6).
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.03x10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   Nt
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:11
D7S820:9,12
D16S539:11,12
VWA:17
TH01:9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   0%
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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