JCRB1522 YS-1

Cell information

Important Notice(s)

本細胞株の民間企業への分譲について

On the MTA of this cell line with Kyoto University (for profit organizations in Japan)

本細胞株の使用について

Announcement of unavailability of this cell line to overseas researcher outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1522 Cell Name   YS-1
Profile   Human primmary cell line established from stomach (tumor infiltrating fibroblast). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57-year-old
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   tumor infiltrating fibroblast, doubling time: 29h
Classify   primary Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2013
Medium   Ham's F12 medium + DMEM (1 to 1 mix) with 5% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio 1/2 Race   
CO2 Conc.   5% Tissue Sampling   stomach
Virus Summary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control] Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)
Tissue Type   
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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_VN81
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LOT Information

Cell No.   JCRB1522 Cell Name   YS-1
LOT No.   03212013 Lot Specification   distribution
Medium   DMEM and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   NT
Cell Density at Seeding   4-8x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(1/3split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.37x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   p10 PDL   U+7.14
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:10,13
D7S820:9,11
D16S539:9,12
VWA:16,18
TH01:6,7
AM:X,Y
TPOX:8
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   NT
Additional information   YS-1 cells display limited life span. The growth of this cell line arrests around 45 passages.
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