JCRB1529 NCI-H2009-Luc

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1529 Cell Name   NCI-H2009-Luc
Profile   Luciferase stably expressing cell line (NCI-H2009; Human lung adenocarcinoma cell line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   69-year-old
Identity   available Tissue for Primary Cancer   lung
Case history   adenocarcinoma Metastasis   Yes
Tissue Metastasized   lymph node Genetics   pMSCV-luc transfected. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   puromycin-resistant
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   DMEM:F12 medium with 0.005 mg/ml Insulin, 0.01 mg/ml Transferri, 30nM Sodium selenite (final conc.), 10 nM Hydrocortisone (final conc.), 10 nM beta-estradiol (final conc.), extra 2mM L-glutamine (for final conc. of 4.5 mM), and 7.5% fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage    Race   Caucasian
CO2 Conc.   5% Tissue Sampling   
Tissue Type   lung

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4Y15
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LOT Information

Cell No.   JCRB1529 Cell Name   NCI-H2009-Luc
LOT No.   03292013 Lot Specification   distribution
Medium   DMEM/ F12(1/1) medium with 0.005 mg/ml insulin ,0.01mg/ml transferrin,30nM Sodium selenite,10nM hydrocortisone,10nM beta-estradiol,4.5mM L-glutamine and 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.4-2.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/10).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.44x10^6
Viability at cell freezing (%)   99.3 Antibiotics Used   
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:12
D7S820:9,11
D16S539:12
VWA:14
TH01:9.3
AM:X
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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