JCRB1537 LoVo-Luc#2

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)について / Ordering Luc cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1537 Cell Name   LoVo-Luc#2
Profile   Luciferase stably expressing cell line (Lovo; Human colorectal adenocarcinoma cell line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   56-year-old
Identity   available Tissue for Primary Cancer   
Case history   colon, adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   pMSCV-luc transfected. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Carcinoembryonic antigen-producing
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   Ham's F12 medium with 20% fetal calf serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio = 1/2 - 1/10 Race   
CO2 Conc.   5% Tissue Sampling   colon
Virus Summary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control] Virus Tested   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)
Tissue Type   
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LOT Information

Cell No.   JCRB1537 Cell Name   LoVo-Luc#2
LOT No.   07092013 Lot Specification   distribution
Medium   Ham's F12 medium with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.17-1.8x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.55x10^6
Viability (%)   98.7 Antibiotics Used   
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,11
D7S820:9.3,10,11
D16S539:9,12
VWA:17,18
TH01:9.3
AM:X,Y
TPOX:8,9
CSF1PO:11,13,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Additional information   
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