JCRB1565 Skipper

Cell information

Important Notice(s)

Distribution(lot no.03132014)は、1vialあたりの細胞数が非常に少ないため、24well plateで1wellに解凍して下さい

Cell type:human iPS cells (View Pricing Information)

JCRB No.   JCRB1565 Cell Name   Skipper
Profile   ES-like cells derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   14 week fetus
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   ES-like cells derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc).
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   
Classify   transformed Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2008
Medium   human ES medium (iPSellon (cardio inc.) ) Methods for Passages   Subculture once a week by mechanical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.)
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D954
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LOT Information

Cell No.   JCRB1565 Cell Name   Skipper
LOT No.   03132014 Lot Specification   distribution
Medium   Culture medium for human iPS cells (attachmentA) ,FGF-2 10ng/ml Temperature   37 C
Cell Density at Seeding    Methods for Passages   Mechanical Passage/0.1%Dispase(Dispase II neutral protease,grade II,Roche 04942078001).(Split ratio=1/1-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.5x10^4
Viability at cell freezing (%)   33.0 Antibiotics Used   
Passage Number   p4+8+6(p4+14) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:11,14
D7S820:10,11
D16S539:9,11
VWA:15
TH01:8
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO,10ng/ml FGF-2,10uM ROCK inhibitor in Culture medium for human iPS cells(attachmentB) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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