JCRB1567 NP 3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1567 Cell Name   NP 3
Profile   Human cell line derived from human glioma. Other Name   NB3972
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64-year-old
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   P53 mutation, P14/P16 deletion, PTEN mutation
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   -
Classify   tumor Established by   Ymazaki, K. and Kumanishi, T.
Registered by   Kumanishi, T. Regulation for Distribution   
Comment    Year   2013
Medium   DMEM with 10% FBS Methods for Passages   0.02% Trypsin + 0.01% EDTA
Cell Number on Passage   1:3 - 1:5 / week Race   Japanese
CO2 Conc.    Tissue Sampling   brain
Tissue Type   anaplastic glioma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1R16

LOT Information

Cell No.   JCRB1567 Cell Name   NP 3
LOT No.   12122013 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.7-6.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.02% trypsin and 0.01% EDTA.Split ratio=1/3-1/5 once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information