JCRB1646 HSC-3

Cell information

Important Notice(s)

サル由来細胞株の培養方法(特に解凍操作)に関して

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1646 Cell Name   HSC-3
Profile   Peripheral blood lymphocyte cell line of cynomolgus monkey (immortalized by Herpesvirus saimiri). Other Name   
Animal   monkey, Cynomolgus Strain   
Genus   Macaca Species   fascicularis
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Akari, H.
Registered by   Akari, H. Regulation for Distribution   
Comment    Year   2015
Medium   RPMI1640 with 10% fetal bovine serum, IL-2 (Interleukin-2, human). Methods for Passages   Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood lymphocytes
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG03
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LOT Information

Cell No.   JCRB1646 Cell Name   HSC-3
LOT No.   11102015 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and IL-2(50 U/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   2.0-7.0x10^5 cells/ml Methods for Passages   Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.34x10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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