JCRB1659 cyfH-5

Cell information

Cell type:primate ES/iPS cells (View Pricing Information)

JCRB No.   JCRB1659 Cell Name   cyfH-5
Profile   Cynomolgus monkey induced pluripotent stem cells derived from fetal liver. Other Name   
Animal   monkey, Cynomolgus Strain   
Genus   Macaca Species   fascicularis
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   Induced pluripotent stem (iPS) cells established by introduction of the transgenes POU5F1 (also known as Oct3/4), SOX2, KLF4 and c-MYC.
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   
Classify   transformed Established by   Shimozawa, N.
Registered by   Shimozawa, N. Regulation for Distribution   
Comment    Year   2015
Medium   DMEM/F12 (1:1) supplemented with 20% KSR, 1% GlutaMax, 0.1 mM 2-ME, 1% NEAA, 10 ng/mL hLIF, and 4 ng/mL human recombinant basic fibroblast growth factor (hbFGF) Methods for Passages   0.1% collagenase in DMEM and divided mechanically into small clusters with pipetting.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JF99
Reference
Pubmed id:23792767 Cynomolgus monkey induced pluripotent stem cells established by using exogenous genes derived from the same monkey species.
Shimozawa N,Ono R,Shimada M,Shibata H,Takahashi I,Inada H,Takada T,Nosaka T,Yasutomi Y
Differentiation. 2013 Apr-Jun;85(4-5):131-9




LOT Information

Cell No.   JCRB1659 Cell Name   cyfH-5
LOT No.   01262015 Lot Specification   distribution
Medium   DMEM and Ham's F12 medium(1/1) with 20% SSR(Wako),1% GlutaMax,1% NEAA,0.1mM 2-mercaptoethanol,10ng/ml hu r LIF and 4ng/ml hu r bFGF on X-irradiated MEF(ICR) feeder cells Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Cells were treated with 0.1% collagenase Type IV (WAKO 590-02793) and divided into small clusters with EZ-Passage Tool and pipetting.(1/6-1/10 Split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   NT
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   p20+3 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   STEM-CELLBANKER(ZENOAQ) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Mitomycin C -treated or X-irradiated MEFs are used as feeders. 1vial is 0.5ml. Type of ampoules is plastic.
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