JCRB1698 cynS-1
Cell information
Cell type：primate ES/iPS cells (View Pricing Information)
| JCRB No. | JCRB1698 | Cell Name | cynS-1 |
|---|---|---|---|
| Profile | Cynomolgus monkey induced pluripotent stem cells derived from newborn skin. | Other Name | |
| Animal | monkey, Cynomolgus | Strain | |
| Genus | Macaca | Species | fascicularis |
| Sex | F | Age | |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Induced pluripotent stem (iPS) cells established by introduction of the transgenes POU5F1 (also known as Oct3/4), SOX2, KLF4 and c-MYC. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | |
| Classify | transformed | Established by | Shimozawa, N. |
| Registered by | Shimozawa, N. | Regulation for Distribution | Restricted by Washington Treaty |
| Comment | Year | 2015 | |
| Medium | DMEM/F12 (1:1) supplemented with 20% KSR, 1% GlutaMax, 0.1 mM 2-ME, 1% NEAA, 10 ng/mL hLIF, and 4 ng/mL human recombinant basic fibroblast growth factor (hbFGF) | Methods for Passages | 0.1% collagenase in DMEM and divided mechanically into small clusters with pipetting. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:23792767 | Cynomolgus monkey induced pluripotent stem cells established by using exogenous genes derived from the same monkey species. Shimozawa N,Ono R,Shimada M,Shibata H,Takahashi I,Inada H,Takada T,Nosaka T,Yasutomi Y Differentiation. 2013 Apr-Jun;85(4-5):131-9 |
LOT Information
| Cell No. | JCRB1698 | Cell Name | cynS-1 |
|---|---|---|---|
| LOT No. | 08042016 | Lot Specification | distribution |
| Medium | DMEM and Ham's F12 medium(1/1) with 20% SSR(Wako),1% GlutaMax,1% NEAA,0.1mM 2-mercaptoethanol,10ng/ml hu r LIF and 4ng/ml hu r bFGF on X-irradiated MEF(ICR) feeder cells | Temperature | 37 C |
| Cell Density at Seeding | NT | Methods for Passages | Cells were treated with 0.1% collagenase Type IV (WAKO 590-02793) and divided into small clusters with EZ-Passage Tool and pipetting.(1/6-1/30 Split) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | NT |
| Viability at cell freezing (%) | NT | Antibiotics Used | |
| Passage Number | p17+3 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | STEM-CELLBANKER(ZENOAQ) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | Mitomycin C -treated or X-irradiated MEFs are used as feeders. 1vial is 0.5ml. Type of ampoules is plastic. |