JCRB1703 ASF-4-5L2

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)


Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1703 Cell Name   ASF-4-5L2
Profile   Normal diploid fibroblast with limeted life span. The ASF-4-1, ASF-4-2, ASF-4-3, ASF-4-4 and the ASF-4-5 lines were established from skins of the same donor at different ages. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   67.4-year-old
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   skin fibroblast, diploid (M)
Life Span   finite Crisis PDL   39.7 PDL
Morphology   fibroblast-like Character   normal fibroblast
Classify   primary Established by   Kaji, K.
Registered by   Kazuhiko Kaji Regulation for Distribution   MTA
Comment    Year   2016
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Subculture every 7 days. Split ratio, 1/4. 0.25% trypsin, 10-15 min.
Cell Number on Passage   5,000 to 10,000 cells/cm^2 Race   Japanese
CO2 Conc.   5% Tissue Sampling   skin
Tissue Type   skin

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG43

LOT Information

Cell No.   JCRB1703 Cell Name   ASF-4-5L2
LOT No.   09052016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(SIGMA12J396). Temperature   37 C
Cell Density at Seeding   0.8-1.7x10^4 cells/cm^2 Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. .
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.04x10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   p4* PDL   14.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information