JCRB1718 H1781/CMV-Luc#6

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)について / Ordering Luc cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1718 Cell Name   H1781/CMV-Luc#6
Profile   Luciferase stably expressing cell line (H1781; human;adenocarcinoma; bronchoalveolar carcinoma). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   66-year-old
Identity   available Tissue for Primary Cancer   lung
Case history   stage 3B,adenocarcinoma; bronchoalveolar carcinoma). Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected H1781 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology    Character   
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2016
Medium   The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. Methods for Passages   Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution.A subcultivation ratio of 1:2 to 1:4 is recommended.Medium Renewal: Every 2 to 3 days
Cell Number on Passage    Race   Caucasian
CO2 Conc.   5% Tissue Sampling   lung; derived from metastatic site:
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG95
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LOT Information

Cell No.   JCRB1718 Cell Name   H1781/CMV-Luc#6
LOT No.   12232016 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.0-1.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   87.2 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:14
D7S820:9,10
D16S539:8
VWA:14
TH01:9
AM:X
TPOX:10,11
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.3
Additional information   
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