JCRB1722 MN20

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1722 Cell Name   MN20
Profile   Human fibroblast cell Line with mutation of LDL receptor Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   N
Tissue Metastasized    Genetics   LDL receptor mutation (intron 15 - exon 18, Alu-Alu recombination between Intron 15 and Exon 18)
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   LDL receptor mutation, Deletion of Exon 16-18, Homozygote (born from consanguineous marriage), Internalization defective type
Classify   primary Established by   Yasuko Miyake
Registered by   Ken-ichi Hirano Regulation for Distribution   
Comment    Year   2016
Medium   DMEM with 10% FBS Methods for Passages   Trypsin-EDTA. split ratio = 1:1. Once/3-4 days.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Virus Summary    [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_UF91
Reference
Pubmed id:3818645 Alu-Alu recombination deletes splice acceptor sites and produces secreted low density lipoprotein receptor in a subject with familial hypercholesterolemia.
Lehrman MA,Russell DW,Goldstein JL,Brown MS
J Biol Chem. 1987 Mar 5;262(7):3354-61
Pubmed id:3312181 Cells of an internalization-defective familial hypercholesterolemia mutant secrete low density lipoprotein receptors.
Miyake Y,Funahashi T,Yamamoto A
J Biochem. 1987 Jun;101(6):1355-60
Pubmed id:6272292 Homozygous familial hypercholesterolemia mutant with a defect in internalization of low density lipoprotein.
Miyake Y,Tajima S,Yamamura T,Yamamoto A
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5151-5

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LOT Information

Cell No.   JCRB1722 Cell Name   MN20
LOT No.   02152017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.15-3.32x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.27x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p9 PDL   13.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11,13
D7S820:10,11
D16S539:10,12
VWA:16
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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