JCRB1762 Akata-EBV-P

Cell information

Important Notice(s)

JCRB1762 Akata-EBV-PのEBV潜伏感染について

Cell type:virus production cells (View Pricing Information)

JCRB No.   JCRB1762 Cell Name   Akata-EBV-P
Profile   Epstein-Barr virus (EBV)-positive cell line established from a Japanese patient with Burkitt’s lymphoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4-year-old
Identity   not done Tissue for Primary Cancer   
Case history   Burkitt’s lymphoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Kenzo Takada
Registered by   Kenzo Takada Regulation for Distribution   
Comment    Year   2017
Medium   RPMI1640 with 10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   blood
Virus Summary   EBV(+) [-/negative, +/positive, nt/not tested,GAPDH is positive control] Virus Tested   GAPDH(+),CMV(-),EBV(+),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_VN87
Reference
Pubmed id:8057484 Isolation of Epstein-Barr virus (EBV)-negative cell clones from the EBV-positive Burkitt's lymphoma (BL) line Akata: malignant phenotypes of BL cells are dependent on EBV.
Shimizu N,Tanabe-Tochikura A,Kuroiwa Y,Takada K
J Virol. 1994 Sep;68(9):6069-73

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LOT Information

Cell No.   JCRB1762 Cell Name   Akata-EBV-P
LOT No.   05252018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.0-3.0x10^5 cells/ml Methods for Passages   dilution 1/3-1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.0x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8,12
D7S820:10,12
D16S539:10,11
VWA:14,16
TH01:9
AM:X
TPOX:8,11
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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