JCRB1776 OCUG-1-Luc#5

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines


Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1776 Cell Name   OCUG-1-Luc#5
Profile   Luciferase stably expressing cell line (OCUG-1; human gall bladder carcinoma). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   43-year-old
Identity   not done Tissue for Primary Cancer   liver, gallbladder
Case history   gallbladder carcinoma Metastasis   Y
Tissue Metastasized    Genetics   pLVSIN-luc transfected. Luciferase stably expressing cell line. Chromosome number distribute in a broad range from 52 to 139.
Life Span    Crisis PDL   
Morphology   clusters with monolayer cells Character   Only TA-4 antigen detected in the spent medium while high levele SLX, CA19-9, SPan-1 and TA-4 were observed in the serum of patient. Puromycin-resistant.
Classify   tumor Established by   Yamada,N. et.al.
Registered by   Takashi Murakami Regulation for Distribution   
Comment   Cells are tumorigenic in nude mice. Poorly defferentiated adenocarcinoma. Year   
Medium   Dulbecco's modified Eagle's medium 0.5mM pyruvate, 2mM glutamine and 10% fetal calf serum. Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   malignant peritoneal effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_XD19

LOT Information

Cell No.   JCRB1776 Cell Name   OCUG-1-Luc#5
LOT No.   12142018 Lot Specification   distribution
Medium   DMEM(GIBCO) with 0.5mM Sodiumpyruvate,2mM L-Glutamine and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.3-1.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   92.67 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information