JCRB1809 Vero/hSLAM

Cell information

Important Notice(s)

About the exportation of Vero/hSLAM - CITES regulated cell line -

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1809 Cell Name   Vero/hSLAM
Profile   Stable expression clone of Vero cell line with human signaling lymphocytic activation molecule (hSLAM). Other Name   
Animal   monkey, African green Strain   
Genus    Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Measles virus (MV) highly susceptible strain
Classify   transformed Established by   Yanagi, Y.
Registered by   Makoto Takeda Regulation for Distribution   
Comment    Year   
Medium   7% FBS-DMEM, Penicillin(100 unit/mL)、Streptomycin (100 ug/mL), Geneticin (G418)(0.4 mg/mL) Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_L037
Pubmed id:11287589Measles viruses on throat swabs from measles patients use signaling lymphocytic activation molecule (CDw150) but not CD46 as a cellular receptor.
Ono N,Tatsuo H,Hidaka Y,Aoki T,Minagawa H,Yanagi Y
J Virol. 2001 May;75(9):4399-401


LOT Information

Cell No.   JCRB1809 Cell Name   Vero/hSLAM
LOT No.   10082019 Lot Specification   distribution
Medium   DMEM(GIBCO) with 7% heat inactivated fetal bovine serum(SIGMA12J396) and G418(0.4mg/mL) Temperature   37 C
Cell Density at Seeding   3.3x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA at 37 C(10-13 min)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.14x10^6
Viability at cell freezing (%)   95 Antibiotics Used   G418
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97
Additional information