JCRB1819 VeroE6/TMPRSS2

Cell information

Important Notice(s)

On the commercial use of Vero/TMPRSS2

Mycoplasmal contamination --> confirmed the elimination of mycoplasmas / マイコプラズマ感染に関して VeroE6/TMPRSS2 --> 完全に除染を確認

About the exportation of VeroE6/TMPRSS2 - CITES regulated cell line -

To customers in Europe [VeroE6/TMPRSS2]

Cell type:CITES regulated cells (View Pricing Information)

JCRB No.   JCRB1819 Cell Name   VeroE6/TMPRSS2
Profile   VeroE6 cells expressing the transmembrane serine protease TMPRSS2. Other Name   
Animal   monkey, African green Strain   
Genus   Chlorocebus Species   sabaeus
Sex   F Age   
Identity    Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   Cells were transfected with pAP3neo encoding human TMPRSS2 (plasmid vector)
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   VeroE6 cells expressing the transmembrane serine protease TMPRSS2.
Classify   transformed Established by   Takeda, M.
Registered by   Makoto Takeda Regulation for Distribution   
Comment   The female origin was determined by karyotype analysis with FISH in Vero. The scientific name of African green monkey was described as Cercopithecus aethiops at Vero cell establishment. Year   
Medium   10% FBS-DMEM, Penicillin (100 unit/mL), Streptomycin (100 ug/mL), Geneticin (G418)(1mg/ml) (at the establishment). Current medium for propagation: DMEM (low glucose) with 10% FBS and 1 mg/mL G418. Methods for Passages   Treatment with trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_YQ49
Reference
Pubmed id:32165541Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Matsuyama S,Nao N,Shirato K,Kawase M,Saito S,Takayama I,Nagata N,Sekizuka T,Katoh H,Kato F,Sakata M,Tahara M,Kutsuna S,Ohmagari N,Kuroda M,Suzuki T,Kageyama T,Takeda M
Proc Natl Acad Sci U S A. 2020 Mar 31;117(13):7001-7003
Pubmed id:31013314Consensus and variations in cell line specificity among human metapneumovirus strains.
Nao N,Sato K,Yamagishi J,Tahara M,Nakatsu Y,Seki F,Katoh H,Ohnuma A,Shirogane Y,Hayashi M,Suzuki T,Kikuta H,Nishimura H,Takeda M
PLoS One. 2019;14(4):e0215822


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Pubmed id:32165541 Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Matsuyama S,Nao N,Shirato K,Kawase M,Saito S,Takayama I,Nagata N,Sekizuka T,Katoh H,Kato F,Sakata M,Tahara M,Kutsuna S,Ohmagari N,Kuroda M,Suzuki T,Kageyama T,Takeda M
Proc Natl Acad Sci U S A. 2020 Mar 31;117(13):7001-7003

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LOT Information

Cell No.   JCRB1819 Cell Name   VeroE6/TMPRSS2
LOT No.   02222020 Lot Specification   distribution2
Medium   Dulbecco's modified Eagle's medium with 5% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) and G418(1 mg/mL) Temperature   37 C
Cell Density at Seeding   1.00-1.55x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.50x10^6
Viability at cell freezing (%)   98 Antibiotics Used    G418
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97
Additional information   Mycoplasma has eliminated with Plasmocure (Final concentratre : 50 ug/mL).
Images
Cell No.   JCRB1819 Cell Name   VeroE6/TMPRSS2
LOT No.   04172020 Lot Specification   distribution3
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) and G418(1 mg/mL) Temperature   37 C
Cell Density at Seeding   1.5-2.33x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.48x10^6
Viability at cell freezing (%)   98 Antibiotics Used   G418
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95
Additional information   02212020(seed):Mycoplasma has eliminated with Plasmocure (Final concentratre : 50 ug/mL).
Images
Cell No.   JCRB1819 Cell Name   VeroE6/TMPRSS2
LOT No.   07022020 Lot Specification   distribution4
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) and G418(1 mg/mL) Temperature   37 C
Cell Density at Seeding   1.5-2.18x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.37x10^6
Viability at cell freezing (%)   99 Antibiotics Used   G418
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95
Additional information   02212020(seed):Mycoplasma has eliminated with Plasmocure (Final concentratre : 50 ug/mL).
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