JCRB1831 Vero ΔPVR1

Cell information

Important Notice(s)


About the distribution of cell lines developed by Crispr/Cas9 technology.

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1831 Cell Name   Vero ΔPVR1
Profile   Poliovirus Receptor α1 (GenBank: D12611.1) deficient Vero cell line Other Name   
Animal   monkey, African green Strain   African Green
Genus   Cercopithecus Species   aethiops
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   Poliovirus receptor α1 was deleted by CRISPR/Cas9 technology
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Okemoto-Nakamura Yuko
Registered by   Kentaro Hanada Regulation for Distribution   
Comment    Year   
Medium   Eagle's minimal essential medium with 5% heat-inactivated fetal bovine serum. Methods for Passages   Cells were treated with AccuMax.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B3LR
Pubmed id:33762624Poliovirus-nonsusceptible Vero cell line for the World Health Organization global action plan.
Okemoto-Nakamura Y,Someya K,Yamaji T,Saito K,Takeda M,Hanada K
Sci Rep. 2021 Mar 24;11(1):6746


LOT Information

Cell No.   JCRB1831 Cell Name   Vero ΔPVR1
LOT No.   10052020 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with 5% heat inactivated fetal bovine serum(SIGMA cat.No.172012 LotNo.12J396) Temperature   37 C
Cell Density at Seeding   0.901-1.16x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with AccuMax(Innovative Cell Technologies:Cat No.AM105).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.93x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p15 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information