JCRB1921 TCC-Pan2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1921 Cell Name   TCC-Pan2
Profile   Pancreatic ductal adenocarcinoma. Cell line established from ascites. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   47-year-old
Identity   available Tissue for Primary Cancer   pancreas
Case history   Pancreatic ductal adenocarcinoma. Metastasis   Y
Tissue Metastasized   ascites Genetics   KRAS (G12R) and amplification (16-fold), TP53 (splicing), CDKN2A homozygous deletion.
Life Span   infinite Crisis PDL   
Morphology   round shape Character   floating (mild adherent), Doubling time: 26 hours
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   
Medium   PMI1640 medium + 10% FBS Methods for Passages   dilution
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5% Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Pubmed id:30747829Development and Biological Analysis of a Novel Orthotopic Peritoneal Dissemination Mouse Model Generated Using a Pancreatic Ductal Adenocarcinoma Cell Line.
Yanagihara K,Kubo T,Mihara K,Kuwata T,Ochiai A,Seyama T,Yokozaki H
Pancreas. 2019 Mar;48(3):315-322


LOT Information

Cell No.   JCRB1921 Cell Name   TCC-Pan2
LOT No.   10242022 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (Biowest cat.No. S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.72-6.66x10^4 cells/sq.cm Methods for Passages   Dilution. Cells were suspended after treatment with Cell Dissociation Buffer (Gibco Cat. No.13151-014) or 1 mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.81x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   91.2 Additional information