JCRB9009 A-431

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9009 Cell Name   A-431
Profile   epidermoid carcinoma, high expression of EGF-receptor Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   85
Identity    Tissue for Primary Cancer   epidermoid carcinoma
Case history    Metastasis   
Tissue Metastasized    Genetics   epidermoid carcinoma
Life Span    Crisis PDL   
Morphology    Character   epidermoid carcinoma
Classify    Established by   Giard & Aaronson
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CRL1555. Year   1985
Medium   Dulbecco's modified Eagle's medium with 4.5g/L glucose and fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/3 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   epiderm
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0037
Reference
Pubmed id:2459591 The epidermal growth factor- and interferon-independent effects of double-stranded RNA in A431 cells.
Chapekar MS,Knode MC,Glazer RI
Mol Pharmacol. 1988 Oct;34(4):461-6
Pubmed id:4357758 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
Giard DJ,Aaronson SA,Todaro GJ,Arnstein P,Kersey JH,Dosik H,Parks WP
J Natl Cancer Inst. 1973 Nov;51(5):1417-23
Pubmed id:2458340

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Pubmed id:3526330

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LOT Information

Cell No.   JCRB9009 Cell Name   A-431
LOT No.   080594 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 4.5g/l glucose and 10% fetal bovine serum /Bio cell, 4001776 Temperature   37 C
Cell Density at Seeding   2-3x10^5 cells/ml Methods for Passages   Cells were harvested after the treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.3x10^5
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P28 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   human (NP,G6PD,PepB,MPI,AST)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   F00954 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (M.A.Bioproducts, lot.99965) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin (Difco 1:250).
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   91.9 Antibiotics Used   free
Passage Number   P29 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:9,13
D7S820:10
D16S539:12,14
VWA:15,17
TH01:9
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   022592 Lot Specification   distribution
Medium   D.MEM (lot.120291 with glucose 4.5g/l)+FBS 10 % (J.R. Scientific CO19407) Temperature   37 C
Cell Density at Seeding   1x10^6 Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   24 hr Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   free
Passage Number   P30 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   122589 Lot Specification   distribution
Medium   DMEM ( glucose 4.5g/l ) + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   021086 Lot Specification   distribution
Medium   DMEM ( glucose 4.5g/l ) + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P28 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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