JCRB9012 RAJI

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9012 Cell Name   RAJI
Profile   lymphoma, Burkitt's Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   Burkitt lymphoma
Life Span    Crisis PDL   
Morphology    Character   Burkitt lymphoma
Classify    Established by   Pulvertaft,R.J.V.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 86. Year   1985
Medium   RPMI1640 medium with 10-20% fetal calf serum. Methods for Passages   Dilution. A maximum of 2-3x10^6 cells/ml is obtained.
Cell Number on Passage   split ratio=3-5x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   Burkitt lymphoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0511
Reference
Pubmed id:165958 Biological differences between Epstein-Barr virus (EBV) strains with regard to lymphocyte transforming ability, superinfection and antigen induction.
Menezes J,Leibold W,Klein G
Exp Cell Res. 1975 May;92(2):478-84
Pubmed id:4365093 Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line.
Nyormoi O,Klein G,Adams A,Dombos L
Int J Cancer. 1973 Sep 15;12(2):396-408
Pubmed id:4316788 Differential reactivity of human serums with early antigens induced by Epstein-Barr virus.
Henle W,Henle G,Zajac BA,Pearson G,Waubke R,Scriba M
Science. 1970 Jul 10;169(3941):188-90
Pubmed id:4288580 Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).
Epstein MA,Achong BG,Barr YM,Zajac B,Henle G,Henle W
J Natl Cancer Inst. 1966 Oct;37(4):547-59
Pubmed id:14293790 CHARACTERISTICS AND MODE OF GROWTH OF TISSUE CULTURE STRAIN (EB1) OF HUMAN LYMPHOBLASTS FROM BURKITT'S LYMPHOMA.
EPSTEIN MA,BARR YM
J Natl Cancer Inst. 1965 Feb;34():231-40
Pubmed id:14086209 CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA).
PULVERTAFT JV
Lancet. 1964 Feb 1;1(7327):238-40

Images



LOT Information

Cell No.   JCRB9012 Cell Name   
LOT No.   02012010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.2 - 7.2 x 10^5 cells/ml Methods for Passages   Simple dilution
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   79.4 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   RAJI
LOT No.   042095 Lot Specification   distribution
Medium   RPMI1640 medium with 20% heat inactivated fetal bovine serum (JR Scientific,D010400) Temperature   37 C
Cell Density at Seeding   1.1-2 x 10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   30.8 hr @3x10^6 Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   RAJI
LOT No.   071092 Lot Specification   distribution
Medium   RPMI1640(lot.062992) + 10% FBS (ICN FLOW1010183-063092) Temperature   37 C
Cell Density at Seeding   8.7x10^5 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.52x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   *P6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   RAJI
LOT No.   062791 Lot Specification   distribution
Medium   RPMI1640 + Heat inactivated FBS 20% Temperature   37 C
Cell Density at Seeding   7.5x10^5 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.18x10^6
Viability at cell freezing (%)   93.1 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   RAJI
LOT No.   072789 Lot Specification   distribution
Medium   RPMI1640 + FCS 10-20% Temperature   37 C
Cell Density at Seeding   3-5x10^5/ml Methods for Passages   Dilution. A maximum of 2-3x10^6 cells/ml is obtained.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   RAJI
LOT No.   100786 Lot Specification   distribution
Medium   RPMI1640 + FCS 10-20% Temperature   37 C
Cell Density at Seeding   3-5x10^5/ml Methods for Passages   Dilution. A maximum of 2-3x10^6 cells/ml is obtained.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   92% Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   
LOT No.   052197 Lot Specification   distribution
Medium   RPMI1640 medium with 10-20% fetal calf serum Use 20% FCS at thawing and 10% at log-phase. Temperature   37 C
Cell Density at Seeding   1-3 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   6.1 x 10^6
Viability at cell freezing (%)   83.0 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9012 Cell Name   RAJI
LOT No.   08162019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.2 - 8.8 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   78.0
Additional information   
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