JCRB9018 CHO-K1

Cell information

Important Notice(s)

CHO-K1はプロリン要求性です。必ず指定培地をご利用ください。

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9018 Cell Name   CHO-K1
Profile   subclone of CHO (Chainese hamster ovary) cells Other Name   
Animal   hamster, Chinese Strain   
Genus   Cricetulus Species   griseus
Sex    Age   
Identity    Tissue for Primary Cancer   ovary
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   used for chromosome aberration test
Classify   normal Established by   Puck,T.T.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 61. Year   1985
Medium   Ham's F12 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0214
Reference
Pubmed id:2237640 Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature.
Tsuji H,Matsudo Y,Tsuji S,Hanaoka F,Hyodo M,Hori T
Somat Cell Mol Genet. 1990 Sep;16(5):461-76
Pubmed id:5271752 Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants.
Kao F,Chasin L,Puck TT
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91

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LOT Information

Cell No.   JCRB9018 Cell Name   
LOT No.   06072010 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 0.5 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   fast (<24 hrs) Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   
LOT No.   07262005 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (GIBCO 10099, lot 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 14 hrs Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   CHO-K1
LOT No.   052996 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9018 Cell Name   CHO-K1
LOT No.   020994 Lot Specification   distribution
Medium   Ham's F12 (GIBCO,powdered) with 10% fetal bovine serum (Hyclone lot.11151031) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Floating cells are harvested first, cells are treated with 0.25% trypsin & 0.02% EDTA for5-8min @37C
Doubling Time   21hrs/5% CO2 Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P15 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   CHO-K1
LOT No.   073192 Lot Specification   distribution
Medium   Ham F-12 medium (Nissui powdered,052012/lot.071092) + 10% fetal bovine serum(J.R.Scientific,DO10400) Temperature   37 C
Cell Density at Seeding   1.12x10^6 Methods for Passages   Harvest floating cells and wash with PBS. Dishes are then treated with 0.25% trypsin-0.02% EDTA.
Doubling Time   18 hr. Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P15 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   CHO-K1
LOT No.   081090 Lot Specification   distribution
Medium   Ham's F12 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   8x10^6
Viability at cell freezing (%)   93% Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   CHO-K1
LOT No.   111588 Lot Specification   distribution
Medium   Ham's F12 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   
LOT No.   031799 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin 0.02% EDTA
Doubling Time   ca 23 hrs Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9018 Cell Name   CHO-K1
LOT No.   11172016 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.7 x 10^5 cells/mL Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.3
Additional information   
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