JCRB9019 3T3-Swiss albino

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9019 Cell Name   3T3-Swiss albino
Profile   fibroblast, contact inhibited, (Swiss albino mouse) Other Name   3T3
Animal   mouse Strain   Swiss albino
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   embryo fibroblasts
Life Span    Crisis PDL   
Morphology    Character   embryo fibroblasts, contact-inhibited fibroblasts
Classify    Established by   Todaro,J. & Green,H.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 92. Year   1985
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow culture to be confluent completely.
Cell Number on Passage   3x10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0120
Reference
Pubmed id:4301006 Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
Aaronson SA,Todaro GJ
J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:14104605 TRANSFORMATION OF PROPERTIES OF AN ESTABLISHED CELL LINE BY SV40 AND POLYOMA VIRUS.
TODARO GJ,GREEN H,GOLDBERG BD
Proc Natl Acad Sci U S A. 1964 Jan;51():66-73
Pubmed id:13926482 Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials.
VOGT M,DULBECCO R
Virology. 1962 Jan;16():41-51

Images



LOT Information

Cell No.   JCRB9019 Cell Name   
LOT No.   08282012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   2.0 - 6.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   8.9 x 10^5
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   P123 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   
LOT No.   04232010 Lot Specification   distribution
Medium   Dulbecco's mosified Eagle's medium with 10% calf serum (GIBCO Cat. # 16010, newborn calf serum) Temperature   37 C
Cell Density at Seeding   1.5 - 2.6 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   92.4 Antibiotics Used   free
Passage Number   P123 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   
LOT No.   04052005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.2 x 10^5
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P124 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   3T3-Swiss albino
LOT No.   030496 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (ICN 1059023) Temperature   37 C
Cell Density at Seeding   1.4x10^4 cells/sq.cm. Methods for Passages   Wash cells with 0.25% trypsin & 0.02% EDTA for 10 min at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   P127 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Freezing medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9019 Cell Name   3T3-Swiss albino
LOT No.   020293 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with calf serum10% (Hyclone 2119480, ICN Flow 1859823) Temperature   37 C
Cell Density at Seeding   1.5x10^4cells/sq.cm Methods for Passages   Cells are harvested by 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.1x10^5
Viability at cell freezing (%)   68.3 Antibiotics Used   free
Passage Number   P126 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   3T3-Swiss albino
LOT No.   041293 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (110992) with calf serum (10%) (ICN,Flow, 1059032) Temperature   37 C
Cell Density at Seeding   68.4x10^4cells/dish Methods for Passages   Harvest cells after treatment with 0.25% Trypsin and 0.02 % EDTA. (Min.Inoc.Size=0.4x10^4/Sq.cm.
Doubling Time   NT Cell Number in Vial (cells/1ml)   124.1x10^4
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   P123 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   3T3-Swiss albino
LOT No.   120991 Lot Specification   distribution
Medium   Dulbecco's EMEM + 10 % CS Temperature   37
Cell Density at Seeding   8.8x10^4/m Methods for Passages   Cells were harvested by 0.02 % EDTA and 0.2% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.21x10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P125 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   3T3-Swiss albino
LOT No.   090991 Lot Specification   distribution
Medium   D.EMEM + CS 10% Temperature   37 C
Cell Density at Seeding   5.4x10^5 Methods for Passages   0.02 % EDTA + 0.2 % trypsin
Doubling Time   36 hr Cell Number in Vial (cells/1ml)   1x10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P126 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   3T3-Swiss albino
LOT No.   020588 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow culture to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P119 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9019 Cell Name   
LOT No.   111897 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^5
Viability at cell freezing (%)   83.0 Antibiotics Used   free
Passage Number   P125 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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