JCRB9022 CPAE

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9022 Cell Name   CPAE
Profile   pulmonary artery normal endothelial cell Other Name   CPAE
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex   F Age   
Identity    Tissue for Primary Cancer   vascular system
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   -24
Morphology   epithelial-like Character   pulmonary artery endothelium
Classify   normal Established by   Del Vecchio,P.
Registered by   Mizusawa,H. Regulation for Distribution   Comments: Collagen coated culture dish is required for this cell line.
Comment   Prepared from CCL209. Year   1985
Medium   Eagle's minimal essential medium with 20% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   pulmonary artery
Virus Summary    Virus Tested   
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2877
Images



LOT Information

Cell No.   JCRB9022 Cell Name   
LOT No.   12172003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 20% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1:3.
Doubling Time   D.T. = ca. 1.8 days Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   P23 PDL   11 at bank
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as bovine by NP, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   052395 Lot Specification   distribution
Medium   EMEM with 20% heat-inactivated FBS,100ug/ml heparin(novo-nordiks) & HUVE growth factor (Morinaga,Co) Temperature   37 C
Cell Density at Seeding   1.86x10^4 cells/sq.cm. Methods for Passages   Rinse with PBS(-) and treated with 0.25% trypsin-0.02% EDTA at 37 C for 5-20min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.37x10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   P21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   100992 Lot Specification   distribution
Medium   Eagle's MEM with 20% FBS(Hyclone),100ug/ml heparin& 100-150ug/ml endotherial cell growth supplement. Temperature   37 C
Cell Density at Seeding   9.0x10^4 cells/ml Methods for Passages   Treat cells in dishes by 0.25% trypsin in 0.02% EDEDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   050891 Lot Specification   distribution
Medium   MEM + FCS 20% Temperature   37 C
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   020888 Lot Specification   distribution
Medium   MEM + FCS 20% Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P18 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
CLOSE