JCRB9026 L1210

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9026 Cell Name   L1210
Profile   leukemia, lymphocytic, (DBA/2 mouse) Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   lymphocytic leukemic cells
Life Span    Crisis PDL   
Morphology    Character   lymphocytic leukemic cells
Classify    Established by   Law,E. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL219. Year   1986
Medium   Fisher's medium with 10% horse serum. Methods for Passages   Dilution. A maximum of 1-2 x 10^6/ml is obtainable.
Cell Number on Passage   4-6 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   spleen lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0382
Reference
Pubmed id:6054389Strengthening dietetic management practices through idea-sharing.
Fallon BJ,Watson HW
J Am Diet Assoc. 1967 Nov;51(5):451-2
Pubmed id:13823721Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.
FOLEY GE,DROLET BP,McCARTHY RE,GOULET KA,DOKOS JM,FILLER DA
Cancer Res. 1960 Jul;20():930-9
Pubmed id:18143260Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice.
LAW LW,DUNN TB
J Natl Cancer Inst. 1949 Aug;10(1):179-92




LOT Information

Cell No.   JCRB9026 Cell Name   
LOT No.   09012006 Lot Specification   distribution
Medium   Fischer's medium with 10% horse serum Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 34 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P384 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9026 Cell Name   L1210
LOT No.   083093 Lot Specification   distribution
Medium   Fischer's medium with 10% horse serum (ICN/Flow 0130293) Temperature   37 C
Cell Density at Seeding   1-3x10^5 cells/ml Methods for Passages   Harvest cells and dilute to 5-10x10^4/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   82.5 Antibiotics Used   freee
Passage Number   P383 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   G6PD, PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9026 Cell Name   L1210
LOT No.   020189 Lot Specification   distribution
Medium   Fisher's medium + horse serum 10% Temperature   37
Cell Density at Seeding   4-6 x 10^4/ml Methods for Passages   Dilution. A maximum of 1-2 x 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P383 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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