JCRB9027 KB

Cell information

Important Notice(s)

JCRB9027　KB細胞のHeLa細胞汚染 / KB is HeLa-derivative.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9027 Cell Name   KB
Profile   The cell line KB has been known that this cell line is a subline of the HeLa. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   HeLa-contaminant
Case history    Metastasis   
Tissue Metastasized    Genetics   epidermoid carcinoma, Oral, Hela markers.
Life Span    Crisis PDL   
Morphology    Character   epidermoid carcinoma, Oral, Hela markers.
Classify    Established by   Eagle,H.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 17. KB cells contains HeLa marker chromosomes and exhibits type A G6PD. Nature, 259, 211, 1976; In Vitro, 14, 469, 1978. Year   1986
Medium   Eagle's MEM with non-essential amino acids and Earle's BSS with 10% FBS Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/4 - 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   oral
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0372
Reference
Pubmed id:4285109Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid".
Kit S,Dubbs DR,DeTorres RA,Melnick JL
Virology. 1965 Nov;27(3):453-7
Pubmed id:14215895THE SENSITIVITY OF GRIVET MONKEY KIDNEY CELL LINE BS-C-1 FOR PROPAGATION AND ISOLATION OF CERTAIN HUMAN VIRUSES.
SCHMIDT NJ,LENNETTE EH,SHON CW,DENNIS J
Am J Public Health Nations Health. 1964 Sep;54():1522-30
Pubmed id:13596943Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents.
EAGLE H,FOLEY GE
Cancer Res. 1958 Oct;18(9):1017-25
Pubmed id:13431913Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters.
FOLEY GE,HANDLER AH
Proc Soc Exp Biol Med. 1957 Apr;94(4):661-4
Pubmed id:13350343Sustained propagation of sarcoma of 180 in tissue culture.
FOLEY GE,DROLET BP
Proc Soc Exp Biol Med. 1956 Jun;92(2):347-52
Pubmed id:13322936Viral susceptibility of a human carcinoma cell (strain KB).
EAGLE H,HABEL K,ROWE WP,HUEBNER RJ
Proc Soc Exp Biol Med. 1956 Mar;91(3):361-4
Pubmed id:13254761Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
EAGLE H
Proc Soc Exp Biol Med. 1955 Jul;89(3):362-4

Images



LOT Information

Cell No.   JCRB9027 Cell Name   
LOT No.   01132010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids (0.1 mM) and 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   P369 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9027 Cell Name   KB
LOT No.   100893 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% heat inactivated FBS. Temperature   37 C
Cell Density at Seeding   1.0x10^6 cells/10cm dish Methods for Passages   Cells are washed once with PBS(-) then treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^6
Viability at cell freezing (%)   91.4 Antibiotics Used   free
Passage Number   P367 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9027 Cell Name   KB
LOT No.   012187 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   98% Antibiotics Used   free
Passage Number   P367 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9027 Cell Name   
LOT No.   071296 Lot Specification   distribution
Medium   Eagle's MEM with non-essential amino acids and 10%fetal calf serum (FCS lot; BioWhittaker 3M0581) Temperature   37 C
Cell Density at Seeding   5-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P367 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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