JCRB9031 MOLT-4

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9031 Cell Name   MOLT-4
Profile   leukemia, acute T lymphoblastic Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   19
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   peripheral blood, ALL, T-cell lymphocytes.
Life Span    Crisis PDL   
Morphology    Character   peripheral blood, ALL, T-cell lymphocytes.
Classify    Established by   Minowada, J.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1582. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtainable.
Cell Number on Passage   4-5 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0013
Reference
Pubmed id:3493047T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.
Drexler HG,Gaedicke G,Minowada J
Blut. 1987 Feb;54(2):79-87
Pubmed id:4608784IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes.
van Boxel JA,Buell DN
Nature. 1974 Oct 4;251(5474):443-4

Images



LOT Information

Cell No.   JCRB9031 Cell Name   
LOT No.   121098 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   Dillution
Doubling Time   ca 25 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   89% Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9031 Cell Name   MOLT-4
LOT No.   022795 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (JRHB, 1B1062) Temperature   37 C
Cell Density at Seeding   1.0x10^5 cells/ml Methods for Passages   Dilution to 1-3 x 10^5 cells/ml.
Doubling Time   23.2 hrs Cell Number in Vial (cells/1ml)   5.7x10^6
Viability at cell freezing (%)   84.2 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD:type B of human, LD, NP tested (human)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:12,13
D7S820:8,10,11
D16S539:11,14,15
VWA:17,18
TH01:6,8
AM:XY
TPOX:8
CSF1PO:11,12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9031 Cell Name   MOLT-4
LOT No.   073192 Lot Specification   distribution
Medium   RPMI1640 (lot.070892) + fetal calf serum 10% (FlowLab. 0070893) Temperature   37 C
Cell Density at Seeding   1.4x10^6 Methods for Passages   dilution, split ration 1:2
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   87.1 Antibiotics Used   free
Passage Number   *P5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9031 Cell Name   MOLT-4
LOT No.   090788 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   4-5 x 10^5/ml Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9031 Cell Name   MOLT-4
LOT No.   10102013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FBS; GIBCO Cat. #10099). Temperature   37C
Cell Density at Seeding   approx. 10^5 cells/mL Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtainable.
Doubling Time    Cell Number in Vial (cells/1ml)   9.0 x 10^5 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.0%
Additional information   
Cell No.   JCRB9031 Cell Name   MOLT-4
LOT No.   03132017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   72.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   72.0
Additional information   
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