JCRB9060 MRC-9

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9060 Cell Name   MRC-9
Profile   fetal lung, normal diploid fibroblast Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   fetal lung, diploid female
Life Span    Crisis PDL   
Morphology    Character   fetal lung, diploid female
Classify    Established by   Jacobs,J.P.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 212. Population doubling is 28 at P4. Year   1987
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2629
Reference
Pubmed id:113411 Characteristics of a serially propagated human diploid cell designated MRC-9.
Jacobs JP,Garrett AJ,Merton R
J Biol Stand. 1979 Apr;7(2):113-22

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LOT Information

Cell No.   JCRB9060 Cell Name   MRC-9
LOT No.   061395 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   9.8x10^5 cells/100mm dish Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.25x10^6
Viability at cell freezing (%)   60.0 Antibiotics Used   free
Passage Number   P7 PDL   34PDL
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,11
D7S820:10,11
D16S539:12
VWA:16,17
TH01:6,8
AM:X
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9060 Cell Name   
LOT No.   F00990 Lot Specification   distribution
Medium   Eagle's MEM with NEAA and 10% FCS (BioWhittaker lot 3M0581) Temperature   37
Cell Density at Seeding   1/2-1/6 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8.0 x 10^5
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P7 PDL   ca 33
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by isoenzymology (NP,G6PD,AST)
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9060 Cell Name   MRC-9
LOT No.   022089 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/6 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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