JCRB9061 PA-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9061 Cell Name   PA-1
Profile   Human ovarian teratocarcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   12
Identity   available Tissue for Primary Cancer   teratocarcinoma
Case history    Metastasis   
Tissue Metastasized    Genetics   ascitic fluid cells, ovarian teratocarcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   ascitic fluid cells, ovarian teratocarcinoma
Classify   tumor Established by   Giovanella,B.C.
Registered by    Regulation for Distribution   
Comment   May be prepared from CRL1572. Year   1987
Medium   MEM w/ non essential amino acids + FCS 10% Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/4 - 1/10 Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   ascites
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0479
Reference
Pubmed id:4524119 Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors.
Giovanella BC,Stehlin JS,Williams LJ Jr
J Natl Cancer Inst. 1974 Mar;52(3):921-30

Images



LOT Information

Cell No.   JCRB9061 Cell Name   
LOT No.   01212002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   ca 24 hrs Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P339 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-base medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9061 Cell Name   PA-1
LOT No.   031695 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids & 10% fetal bovine serum/JRHB 1B1062 Temperature   37 C
Cell Density at Seeding   2x10^4 cells/sq.cm Methods for Passages   Rince cells once with PBS(-) and treated with 0.25% Trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P339 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:9,10,13
D7S820:9
D16S539:9,12
VWA:15,17
TH01:7,9
AM:X
TPOX:11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9061 Cell Name   PA-1
LOT No.   092288 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   93% Antibiotics Used   free
Passage Number   P339 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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