JCRB9068 293

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9068 Cell Name   293
Profile   transformed embryonal kidney by adenovirus (type5) Other Name   HEK293
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   transformed embryonal kidney by adenovirus (type5)
Classify   transformed Established by   Graham,F.L.
Registered by   JCRB Cell Bank Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1573. Year   1987
Medium   Eagle's minimal essential medium with 10% heat inactivated horse serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/3 - 1/12 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0045
Reference
Pubmed id:11792820 Identification of essential genes in cultured mammalian cells using small interfering RNAs.
Harborth J,Elbashir SM,Bechert K,Tuschl T,Weber K
J Cell Sci. 2001 Dec;114(Pt 24):4557-65
Pubmed id:11373684 Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
Elbashir SM,Harborth J,Lendeckel W,Yalcin A,Weber K,Tuschl T
Nature. 2001 May 24;411(6836):494-8
Pubmed id:2535739 Comeasurement of simian virus 40 early and late promoter activity in HeLa and 293 cells in the presence of T antigen.
Kelly JJ,Munholland JM,Wildeman AG
J Virol. 1989 Jan;63(1):383-91
Pubmed id:3135533 Heterogeneity of human U1 snRNAs.
Lund E
Nucleic Acids Res. 1988 Jul 11;16(13):5813-26
Pubmed id:664220 Defective transforming capacity of adenovirus type 5 host-range mutants.
Graham FL,Harrison T,Williams J
Virology. 1978 May 1;86(1):10-21
Pubmed id:886304 Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
Graham FL,Smiley J,Russell WC,Nairn R
J Gen Virol. 1977 Jul;36(1):59-74
Pubmed id:841862 Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.
Harrison T,Graham F,Williams J
Virology. 1977 Mar;77(1):319-29


Research results by users. Click!
Pubmed id:31847265 Applicability of Anti-Human Estrogen Receptor β Antibody PPZ0506 for the Immunodetection of Rodent Estrogen Receptor β Proteins.
Ishii H,Otsuka M,Kanaya M,Higo S,Hattori Y,Ozawa H
Int J Mol Sci. 2019 Dec 13;20(24):
Pubmed id:24398693 The C-terminal domain of the long form of cellular FLICE-inhibitory protein (c-FLIPL) inhibits the interaction of the caspase 8 prodomain with the receptor-interacting protein 1 (RIP1) death domain and regulates caspase 8-dependent nuclear factor κB (NF-κB) activation.
Matsuda I,Matsuo K,Matsushita Y,Haruna Y,Niwa M,Kataoka T
J Biol Chem. 2014 Feb 14;289(7):3876-87

Images
Movies



LOT Information

Cell No.   JCRB9068 Cell Name   293
LOT No.   08202010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat-inactivated horse serum, Lot No. described on vial label is mistake. Correctly, 08202010. Temperature   37 C
Cell Density at Seeding   0.9 - 1.4 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture once a week.
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   90.9 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   10172008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat-inactivated horse serum Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture once a week.
Doubling Time   approx. 55 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   07272006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat-inactivated horse serum Temperature   37 C
Cell Density at Seeding   0.5 - 1.0 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 60 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by isoenzymology.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   12272004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat-inactivated horse serum Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA, subculture twice a week.
Doubling Time   ca. 2 days Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   07142003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat-inactivated horse serum (HS lot; GIBCO 1144886) Temperature   37 C
Cell Density at Seeding   1.3 - 1.6 x 10^5 cells/ml with 4-5 days interval. Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = ca 2.4 days Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   89.9 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% inactivated HS in EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   03282000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat-inactivated horse serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 62 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   11182002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat-inactivated horse serum Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% horse serum in EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   10012001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat-inactivated horse serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% inact-horse serum in MEM CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   061496 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9068 Cell Name   293
LOT No.   031296 Lot Specification   distribution
Medium   Details not recorded here Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9068 Cell Name   293
LOT No.   021894 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat inactivated horse serum (ICN Flow,0130293). Temperature   37 C
Cell Density at Seeding   8.5x10^6 cells/10 cm dish Methods for Passages   Cells are treated with 0.1%trypsin & 0.02%EDTA then inoculated into collagen or gelatin coated disks
Doubling Time   24 hrs Cell Number in Vial (cells/1ml)   3.1x10^6
Viability at cell freezing (%)   93.6 Antibiotics Used   free
Passage Number   P36 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP (G6PD not detected)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   102692 Lot Specification   distribution
Medium   Eagle's Minimal Essential Medium (090992) with 10%heat inactivated horse serum (JRS 001223) Temperature   37 C
Cell Density at Seeding   6.1x10^4 cells/ml Methods for Passages   Treated with 0.25% type I collagenase at 37C,10min.then treated with trypsin(.25%)-EDTA(.02%) again.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.1x10^5
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P38 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   041289 Lot Specification   distribution
Medium   MEM + heat inactivated horse serum 10% Temperature   37 C
Cell Density at Seeding   1/3 - 1/12 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P36 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   060397 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat- inactivated horse serum Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   
LOT No.   072899 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat- inactivated horse serum Temperature   37 C
Cell Density at Seeding   2-3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 50 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   04172015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat-inactivated horse serum. Temperature   37 C
Cell Density at Seeding   approx. 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 48 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   81 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8,9
D13S317:12,14
D7S820:11,12
D16S539:9,13
VWA:16,19
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% horse serum (heat-inactivated) - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   81
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   03072016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat-inactivated horse serum. Temperature   37 C
Cell Density at Seeding   0.3 - 2.3 x 10^5 cells/mL Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   83.1 Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% heat-inactivated horse serum - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   83.1
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   03312017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated horse serum(BIOSCIENCE Lot No. 562126 JRH) Temperature   37 C
Cell Density at Seeding   3.0-3.5 x 10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P40 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,9
D13S317:12,14
D7S820:11,12
D16S539:9,13
VWA:16,19
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.6
Additional information   
Images
Cell No.   JCRB9068 Cell Name   293
LOT No.   09212017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated horse serum. Temperature   37 C
Cell Density at Seeding   5 - 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   94.5 Antibiotics Used   free
Passage Number   P42 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% horse serum - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.5
Additional information   
Cell No.   JCRB9068 Cell Name   293
LOT No.   11112019 Lot Specification   distribution
Medium   Eagle's minimum essential medium (GIBCO) with 10% heat inactivated horse serum (GIBCO:Cat.No.16050-122,Lot No.1861236) Temperature   37 C
Cell Density at Seeding   1.34-1.96x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.52x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p40 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,9
D13S317:12,14
D7S820:11,12
D16S539:9,13
VWA:16,19
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96
Additional information   
Images
CLOSE