JCRB9071 Daudi

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9071 Cell Name   Daudi
Profile   lymphoma, Burkitt's Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   16
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   Burkitt lymphoma
Life Span    Crisis PDL   
Morphology    Character   Burkitt's lymphoma
Classify    Established by   Klein,E. & Klein,G.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL213. Year   1987
Medium   RPMI1640 medium with 20% fetal calf serum Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml .
Cell Number on Passage   5-7 x 10^5/ml Race   
CO2 Conc.   5 % Tissue Sampling   Burkitt lymphoma
Virus Summary   tested Virus Tested   CMV(-), EBV(+), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested], confirmed.
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0008
Reference
Pubmed id:201572 Chromosome banding, isoenzyme studies and determination of Epstein-Barr virus DNA content on human Burkitt lymphoma/mouse hybrids.
Spira J,Povey S,Wiener F,Klein G,Andersson-Anvret M
Int J Cancer. 1977 Dec 15;20(6):849-53

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LOT Information

Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   01292009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   0.6-1.3x10^5 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.24x10^6
Viability at cell freezing (%)   99.8 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,13
D13S317:11,12
D7S820:8,10
D16S539:10,12
VWA:15,17
TH01:6,7
AM:X,Y
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9071 Cell Name   
LOT No.   09262003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   Dilution with subcultivation interval 2-3 days.
Doubling Time   D.T. = 29 hrs Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   92.4 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   051796 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   120694 Lot Specification   distribution
Medium   RPMI1640 medium with 20% heat inactivated fetal bovine serum (Hyclone, 11151031) Temperature   37 C
Cell Density at Seeding   3-4x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   87.9 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   100992 Lot Specification   distribution
Medium   RPMI1640 medium (081792) with 20% heat inactivatedfetal bovine serum (JRH Bioscience 1B1062) Temperature   37 C
Cell Density at Seeding   1.7x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   *P4 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   022791 Lot Specification   distribution
Medium   RPMI1640 + FCS 20% Temperature   37 C
Cell Density at Seeding   5-7 x 10^5/ml Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P6 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   021288 Lot Specification   distribution
Medium   RPMI1640 + FCS 20% Temperature   37
Cell Density at Seeding   5-7 x 10^5/ml Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   04242013 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal calf serum (FBS: GIBCO Cat. #10099) Temperature   37 C
Cell Density at Seeding   2.7 - 6.5 x 10^5/ml Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml .
Doubling Time    Cell Number in Vial (cells/1ml)   6.8 x 10^5 cells/mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.1
Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   09062017 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 1.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   83.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   83.0
Additional information   
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