JCRB9074 G-361

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9074 Cell Name   G-361
Profile   malignant melanoma, skin Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   melanoma
Case history    Metastasis   
Tissue Metastasized    Genetics   malignant melanoma, melanin production
Life Span    Crisis PDL   
Morphology    Character   malignant melanoma, melanin production
Classify    Established by   Peebles,P.T.
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1424. Year   1986
Medium   McCoy's 5a + FCS 10 % Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/3 - 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Tissue Type   
Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1220
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LOT Information

Cell No.   JCRB9074 Cell Name   
LOT No.   07092004 Lot Specification   distribution
Medium   McCoy's 5a medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   34 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability (%)   96.4 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - McCoy's 5a CO2 Conc.   5%
RFLP    Additional information   
Cell No.   JCRB9074 Cell Name   G-361
LOT No.   022895 Lot Specification   distribution
Medium   McCoy-5A medium(ICN,Biomedicals) with 10% heat inactivated fetal bovine serum (JRHB,1B1062) Temperature   37 C
Cell Density at Seeding   1.5x10^4 cells/sq.cm Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA for 3-5 min. at 37 C.
Doubling Time   20.4 hrs Cell Number in Vial (cells/1ml)   1.64x10^6
Viability (%)   95.7 Antibiotics Used   free
Passage Number   P23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD:type B, LD, NP tested (human)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8,11
D16S539:15
VWA:15,17
TH01:7,9
AM:X
TPOX:8,10
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Images
Cell No.   JCRB9074 Cell Name   G-361
LOT No.   012392 Lot Specification   distribution
Medium   McCoy 5A (lot#Flow Laboratories,1022271.) + F.B.S.10% (lot#Cell Culture Laboratories,00939-01) Temperature   37C
Cell Density at Seeding   1.34x10^6 Methods for Passages   0.25%Tripsin + 0.02% EDTA
Doubling Time   20hr Cell Number in Vial (cells/1ml)   4.38x10^6
Viability (%)   89% Antibiotics Used   free
Passage Number   P24 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB9074 Cell Name   G-361
LOT No.   061687 Lot Specification   distribution
Medium   McCoy's 5a + FCS 10 % Temperature   37
Cell Density at Seeding   1/3 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability (%)   NT Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Additional information   
Cell No.   JCRB9074 Cell Name   G-361
LOT No.   12192013 Lot Specification   distribution
Medium   McCoy's 5a medium with 10% FBS (GIBCO Cat. #10091) Temperature   37C
Cell Density at Seeding   2.4 - 4.7 10^4 cells/mL Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6 (cells/1
Viability (%)    Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO CO2 Conc.   5%
RFLP    Additional information   
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